Kutulutsa DNA ya dothi/ndowe mwachangu komanso mwangwiro komanso mosavuta pogwiritsa ntchito Big Fish Sequence

Dothi, monga malo osiyanasiyana okhala ndi zachilengedwe, lili ndi zinthu zambiri zamoyo, kuphatikizapo mitundu yosiyanasiyana ya tizilombo monga mabakiteriya, bowa, mavairasi, cyanobacteria, actinomycetes, protozoa ndi nematodes. Pokhala ndi zochita zambiri za kagayidwe kachakudya komanso zinthu za thupi, amachita gawo lofunika kwambiri pakuzungulira michere ya nthaka ndipo ndi ofunikira pochotsa zinthu zodetsa nthaka. Monga amodzi mwa malo osiyanasiyana kwambiri padziko lapansi, maphunziro a zamoyo zamamolekyulu pa nthaka ndi ofunika kwambiri pazamoyo. Munjira iyi, kupeza DNA ya tizilombo kuchokera ku zitsanzo za nthaka ndi gawo loyamba pakufufuza nthaka komanso gawo lofunika kwambiri kuti mayeso otsatira apite patsogolo. Komabe, kuwonjezera pa zinthu zambiri za tizilombo, nthaka nthawi zambiri imakhala ndi zinthu zambiri za metabolites (humic acid, xanthic acid ndi zinthu zina za humic), zomwe zimatha kuyeretsedwa mosavuta pamodzi ndi nucleic acid panthawi yochotsa nucleic acid, zomwe zimakhudza PCR yotsika komanso njira yotsatirira.Nsomba YaikuluSequencing Soil and Ndowe Genomic DNA Purification Kit imatha kutulutsa DNA yeniyeni komanso yochuluka kwambiri kuchokera ku zitsanzo zokhala ndi humus monga ndowe za nthaka, zomwe zimathandiza kwambiri pa kafukufuku wokhudza kusiyanasiyana kwa tizilombo toyambitsa matenda m'nthaka.