Mwachangu komanso mwangwiro, dothi losavuta / ndowe za DNA zochotsa ndi Big Fish Sequence

Nthaka, monga zachilengedwe zosiyanasiyana, imakhala ndi tizilombo toyambitsa matenda, kuphatikizapo mitundu yambiri ya tizilombo toyambitsa matenda monga mabakiteriya, bowa, mavairasi, cyanobacteria, actinomycetes, protozoa ndi nematodes. Pokhala ndi zochitika zosiyanasiyana za kagayidwe kachakudya ndi mphamvu za thupi, zimagwira ntchito yofunika kwambiri pakuyenda kwa michere ya nthaka ndipo ndizofunikira pakuchotsa zowononga nthaka. Monga amodzi mwa malo omwe ali ndi mitundu yosiyanasiyana yazachilengedwe padziko lapansi, maphunziro achilengedwe a mamolekyulu a dothi ali ndi tanthauzo lapadera. Pochita izi, kupeza tizilombo tating'onoting'ono ta DNA kuchokera ku dothi ndi sitepe yoyamba pa kafukufuku wa nthaka ndi sitepe yofunikira kwambiri pakuchita bwino kwa kuyesa kwapansi. Komabe, kuwonjezera pazambiri za tizilombo tating'onoting'ono, nthaka nthawi zambiri imakhala ndi ma metabolites ambiri (humic acid, xanthic acid ndi zinthu zina za humic), zomwe zimatha kuyeretsedwa mosavuta pamodzi ndi ma nucleic acid panthawi ya nucleic acid m'zigawo, zomwe zimakhudza kutsika kwa PCR ndi kutsatizana.Nsomba ZazikuluKutsata Dothi ndi Faecal Genomic DNA Purification Kit kumatha kutulutsa DNA yoyera komanso yokhazikika kwambiri kuchokera ku zitsanzo zokhala ndi humus monga ndowe za dothi, zomwe zimathandiza kwambiri pakufufuza zamitundu yosiyanasiyana yazachilengedwe.