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Kiti Yoyeretsera DNA ya MagaPure Aquatic Animal Genomic
Chiyambi chachidule
Kiti iyi imagwiritsa ntchito njira yapadera yopangira zinthu zapadera komanso mikanda yamaginito yomwe imalumikizana ndi DNA mwachangu. Imatha kumangirira, kunyamula, kulekanitsa, ndikuyeretsa ma nucleic acid mwachangu, ndipo idapangidwira nyama zam'madzi. Ndi yoyenera kwambiri kutulutsa ndikuyeretsa DNA ya genomic bwino kuchokera ku minofu yosiyanasiyana ya nyama zam'madzi, ndipo imatha kuchotsa zodetsa monga mapuloteni, mafuta, ndi zinthu zina zachilengedwe momwe zingathere. Pogwiritsa ntchito Bigfish Magnetic Bead Nucleic Acid Extractor, ndi yoyenera kwambiri kutulutsa zitsanzo zazikulu zokha. DNA ya genomic yotulutsidwayo ili ndi chiyero chapamwamba komanso yabwino, ndipo ingagwiritsidwe ntchito kwambiri mu PCR/qPCR, NGS, Southern hybridization ndi kafukufuku wina woyesera.
Zinthu zomwe zili mu malonda
◆ Zitsanzo zomwe zimagwiritsidwa ntchito kwambiri: DNA ya genomic ikhoza kutengedwa mwachindunji kuchokera ku zitsanzo zosiyanasiyana za nyama zam'madzi
◆ Yotetezeka komanso yopanda poizoni: Chogwiritsiridwa ntchitochi sichili ndi zinthu zosungunulira poizoni monga phenol ndi chloroform, zomwe zimakhala ndi chitetezo champhamvu.
◆ Makina Odzichitira: Yokhala ndi Bigfish Nucleic Acid Extractor, imatha kutulutsa zinthu zambiri, makamaka yoyenera kutulutsa zitsanzo zazikulu.
◆ Kuyera kwambiri: kungagwiritsidwe ntchito mwachindunji pa kuyesa kwa ma molecular biology monga PCR, kugaya kwa ma enzyme, kusakaniza, ndi zina zotero.
Njira zochotsera
Kusankha: Imwani 25-30 mg ya minofu ya nyama ya m'madzi
Kupera: kupera ndi nayitrogeni yamadzimadzi, kupera ndi chopukusira kapena kumeta
Kugaya chakudya: 56℃ kusamba ndi madzi ofunda
Pa makina: Ikani centrifuge supernatant ndikuyiyika mu mbale kuti muchotse
Magawo aukadaulo
Chitsanzo cha sage: 25-30mg
Kuyera kwa DNA: A260/280≧1.75
Chida chosinthika
Nsomba Yaikulu BFEX-32/BFEX-32E/BFEX-96E
Kufotokozera za malonda
| Dzina la Chinthu | Mphaka. Ayi. | Kulongedza |
| MagaYoyeraGenomic ya Zinyama Zam'madziKiti Yoyeretsera DNA(pphukusi lodzazidwanso) | BFMP21R | 32T |
| MagaYoyeraGenomic ya Zinyama Zam'madziKiti Yoyeretsera DNA (phukusi lodzazitsidwa kale) | BFMP21R1 | 40T |
| MagaYoyeraGenomic ya Zinyama Zam'madziKiti Yoyeretsera DNA (phukusi lodzazitsidwa kale) | BFMP21R96 | 96T |
| RNase A(pchogulitsa) | BFRD017 | 1ml/chubu (10mg/ml) |
Zokonda zachinsinsi