FFPE DNA Purification Kit

Kufotokozera Kwachidule:

Chidacho chimagwiritsa ntchito njira yochepetsera zachilengedwe kuti ichotse DNA yapamwamba kwambiri ya genomic kuchokera ku zitsanzo zokhala ndi parafini, kuthana ndi zoletsa zomwe zimayambitsidwa ndi formalin cross-linking.
Chitsanzocho chimasakanizidwa ndi cell lysis buffer kuti aswe maselo ndikuwonetsa DNA. Chitsanzocho chimasakanizidwa ndi cell lysis buffer kuti athyole maselo ndikuwonetsa DNA. DNA yowonekera imatengedwa ndi maginito bead nanoparticles kupanga mkanda-DNA complex. Mikanda-DNA complexes imasamutsidwa ku Mikanda-DNA ma complexes amasamutsidwa kuti azitsuka buffer kuchotsa zonyansa zambiri. Pomaliza, DNA imachotsedwa ndikubwezeretsedwa ndikuitumiza ku lution buffer.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Zitsanzo

Zigawo za FFPE, midadada ya FFPE, zitsanzo mumayankho okhazikika monga formalin

Zigawo

Chitsanzo

BFMP12R1

BFMP12R16

BFMP12R

Zigawo

Kulongedza

1T40T / zida

16T16T / zida

32T32T / zida

Protease K

400μL x 2

320μL

640μl

Protease solution

(Zodzaza padera)

Rnase A

80μl pa

32μl pa

64μl pa

Njira yothetsera enzyme

(Zodzaza padera)

Buffer FA

12ml pa

5ml ku

10 ml pa

Mkulu wa saline solution

FL

Mu makumi anai 6 - bwino

kukonzekera.

katiriji

40 pcs.

96 pa

zodzaza kale

mbale

2 ma PC

96 pa

zodzaza kale

mbale

2 ma PC.

Wamphamvu denaturant ndi Tris buffer

Mtengo WA

Mkulu wa saline solution

Mtengo WB

Mchere wochepa wa saline

Mtengo DE

Mchere wochepa wa saline

Maginito mikanda

Hydroxy magnetic bead solution

Buku Logwiritsa Ntchito

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1

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DNA Nucleic Acid Extraction Kit
DNA RNA Nucleic Acid Extraction Kit
DNARNA Purification Kit

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