2 × SYBR green qPCR MIX （Yokhala ndi High ROX）

Zinthu zomwe zili mu malonda

Chogulitsachi, 2×SYBR green qPCR MIX, chimabwera mu chubu chimodzi chokhala ndi zinthu zonse zofunika kuti PCR ikule bwino komanso kuizindikira, kuphatikizapo Taq DNA poLymerase, SYBR green I dye, High ROX Reference Dye, dNTPs, Mg2+, ndi PCR buffer.
Utoto wobiriwira wa SYBR ndi utoto wobiriwira wa fluorescent womwe umalumikizana ndi DNA yokhala ndi zingwe ziwiri (DNA yokhala ndi zingwe ziwiri, dsDNA) double helix minor groove region. Utoto wobiriwira wa SYBR I umawala pang'ono mu free state, koma ukalumikizana ndi DNA yokhala ndi zingwe ziwiri, kuwala kwake kumawonjezeka kwambiri. Izi zimapangitsa kuti zikhale zotheka kuyeza kuchuluka kwa DNA yokhala ndi zingwe ziwiri yomwe imapangidwa panthawi ya PCR amplification pozindikira mphamvu ya fluorescence.
ROX imagwiritsidwa ntchito ngati utoto wokonzera kusintha kwa kuwala kosagwirizana ndi PCR, motero kuchepetsa kusiyana kwa malo. Kusiyana kumeneku kungayambitsidwe ndi zinthu zosiyanasiyana, monga vuto la pipette kapena evaporation ya chitsanzo. Zida zosiyanasiyana zowerengera kuwala zili ndi zosowa zosiyanasiyana za ROX, ndipo mankhwalawa ndi oyenera kusanthula kuchuluka kwa kuwala komwe kumafunikira kukonza kwakukulu kwa ROX.