2 × SYBR wobiriwira qPCR MIX (Ndi High ROX)

Zogulitsa

Chogulitsachi, 2 × SYBR chobiriwira qPCR MIX, chimabwera mu chubu chimodzi chomwe chili ndi zigawo zonse zofunika kuti PCR ikulitse ndi kuzindikira, kuphatikizapo Taq DNA poLymerase, SYBR green I dye, High ROX Reference Dye, dNTPs, Mg2 +, ndi PCR buffer.
SYBR green I dye ndi utoto wobiriwira wa fulorosenti womwe umamangiriza ku DNA yamitundu iwiri (DNA yamitundu iwiri, dsDNA) dera la double helix minor groove.SYBR wobiriwira I fluoresces mofooka mu free state, koma kamodzi kamamanga ku DNA yazingwe ziwiri. , fluorescence yake imakulitsidwa kwambiri. Izi zimapangitsa kuti athe kuwerengera kuchuluka kwa DNA yamitundu iwiri yopangidwa pakukulitsa kwa PCR pozindikira mphamvu ya fluorescence.
ROX imagwiritsidwa ntchito ngati utoto wowongolera kukonza kusinthasintha kwa fluorescence kosagwirizana ndi PCR, motero kumachepetsa kusiyana kwa malo. Kusiyanasiyana kotereku kungayambitsidwe ndi zinthu zosiyanasiyana, monga kulakwitsa kwa pipette kapena kutulutsa mpweya. Zida zosiyanasiyana zowerengera za fluorescence zimakhala ndi zosowa zosiyanasiyana za ROX, ndipo izi ndizoyenera kuwunikira ma fluorescence quantification omwe amafunikira kuwongolera kwa High ROX.