Kusiyanasiyana kwa Deep Well Plates mu Laboratory Research

Mbale zakuyandizofunika kwambiri pakufufuza kwa labotale, zomwe zimapereka mayankho osunthika komanso ogwira ntchito osiyanasiyana. Ma mbale a multiwell awa adapangidwa kuti azitengera zitsanzo m'njira yopambana kwambiri, zomwe zimawapangitsa kukhala chida chofunikira pamaphunziro osiyanasiyana asayansi monga genomics, proteinomics, kupeza mankhwala, ndi zina zambiri.

Ubwino waukulu wa mbale zakuya ndikutha kunyamula zitsanzo zambiri. Ma mbalewa ali ndi kuya koyambira 2 mpaka 5 mm ndipo amatha kutengera zitsanzo mpaka 2 ml pachitsime chilichonse, zomwe zimawapangitsa kukhala abwino kwa ntchito zomwe zimafunikira kukonzedwa kwa zitsanzo zambiri. Izi ndizofunikira makamaka pamayesero apamwamba kwambiri pomwe zitsanzo zingapo ziyenera kukonzedwa nthawi imodzi.

Kuphatikiza pa kuchuluka kwa zitsanzo, mbale zakuya zimagwirizana ndi zida zosiyanasiyana za labotale, kuphatikiza makina opangira madzi, ma centrifuges, ndi owerenga mbale. Kugwirizana kumeneku kumalola kuphatikizika kosasunthika mumayendedwe omwe alipo a labotale, kuwongolera njira ndikuwonjezera kuchita bwino. Kaya amagwiritsidwa ntchito pokonzekera zitsanzo, kusungirako kapena kusanthula, mbale zakuya zakuya zimapereka nsanja yodalirika komanso yabwino yochitira zoyesera.

Kuonjezera apo, mbale zozama kwambiri zimapezeka m'mawonekedwe osiyanasiyana, kuphatikizapo 96-, 384-, ndi 1536-makonzedwe bwino, kupereka ochita kafukufuku kusinthasintha malinga ndi zosowa zawo zoyesera. Kusinthasintha kumeneku kumapangitsa kuti mbale zakuya zikhale zoyenera kuti zigwiritsidwe ntchito zosiyanasiyana, kuchokera ku chikhalidwe cha maselo ndi kukula kwa tizilombo toyambitsa matenda kupita ku mapuloteni a crystallization ndi kuyang'anitsitsa.

Mapangidwe a mbale zakuya zachitsime amawapangitsanso kukhala abwino kusungirako ndi kusunga zitsanzo. Kumanga kwawo kolimba komanso kugwirizanitsa ndi njira zosindikizira monga mafilimu omatira ndi ma gaskets a lid zimatsimikizira kukhulupirika kwachitsanzo ndikuchepetsa chiopsezo cha kuipitsidwa. Izi zimapangitsa mbale zakuya zabwino kwambiri zosungirako nthawi yayitali zitsanzo zamoyo, ma reagents ndi mankhwala, kupatsa ofufuza njira yodalirika yoyendetsera zitsanzo.

Kuphatikiza apo, mbale zakuya zozama zimapezeka muzinthu zosiyanasiyana, kuphatikiza polypropylene ndi polystyrene, iliyonse ili ndi maubwino apadera kutengera ntchito. Mwachitsanzo, mbale zakuya za polypropylene zimadziwika chifukwa cha kukana kwawo kwa mankhwala komanso kugwirizana ndi zosungunulira zosiyanasiyana, zomwe zimawapanga kukhala oyenera kugwiritsa ntchito mankhwala osokoneza bongo. Mbali inayi, mbale zakuya za polystyrene, nthawi zambiri zimakondedwa chifukwa cha kuwala kwawo, zomwe zimawapangitsa kukhala abwino kwa mapulogalamu omwe amafunikira kuyang'anitsitsa kapena kufufuza fulorosenti.

Powombetsa mkota,mbale zakuya zachitsimendi chida chofunikira kwambiri pakufufuza kwa labotale, kupereka kusinthasintha, kuchita bwino, komanso kudalirika pamagwiritsidwe osiyanasiyana. Kuthekera kwawo kwachitsanzo chapamwamba, kugwirizana ndi zida za labotale, komanso kusinthasintha kwamawonekedwe ndi zida zimawapangitsa kukhala chuma chamtengo wapatali kwa ofufuza m'magawo osiyanasiyana asayansi. Kaya ndikukonza zitsanzo, kusungirako kapena kusanthula, mbale zakuya zakuya zikupitilizabe kuchitapo kanthu pakupititsa patsogolo kutulukira kwa sayansi ndi luso.