Kutsegula zinsinsi za moyo: Kufunika kwa acid acid

M'munda wa biology biology, kuchotsera kwa acid acids (DNA ndi RNA) ndi gawo lofunika kwambiri, ndikuyika njira yogwiritsira ntchito kufufuza kwa majini ku matenda azachipatala. Zida za nuclectic acizi zowonjezera zamitundu zafuulirazi zidasinthiratu njirayi, ndikupangitsa kukhala kovuta, kodalirika, ndikupezeka kwa ofufuza ndi malo opanga dziko lonse lapansi. Mu blog ino, tikambirana izi, kufunikira kwa zinthu zawo, komanso zomwe zimakhudza kupita patsogolo kwa sayansi.

Kodi zida za nyukiliya ndi ziti?

Nucleic acid pochotsa ma kitsndi zida zopangidwa makamaka kuti zizipatula DNA kapena RNA kuchokera pazitsanzo zosiyanasiyana zachilengedwe, monga magazi, minofu, ma cell, komanso zitsanzo zachilengedwe. Izi zimakhala zimakhala ndi ma protents onse ndi ma protocol omwe amafunikira kuti azitsogolera, kuwonetsetsa kuti ofufuza atha kupeza ma acid apamwamba kwambiri a nuclect ndi kuipitsidwa kochepa.

Njira Yothandizira

Njira yodulira nthawi zambiri imaphatikizapo njira zingapo zazikulu: cell lysis, kuyeretsedwa, ndi khungu.

Cell lysis: gawo loyamba ndikutsegula maselo kuti atulutse ma acid a ma acid. Izi nthawi zambiri zimachitika pogwiritsa ntchito buffer ya lysis yokhala ndi zotchinga ndi ma enzyme omwe amasokoneza ma cell a cell ndi ma priteni.

Kuyeretsa: Pambuyo pa zitsulo zamimba zimatulutsidwa, gawo lotsatira ndikuchotsa zodetsa monga mapuloteni, lipids, ndi zinyalala zina. Ambiri okhala ndi mizere ya sica kapena maginito a maginito kuti amange mosamalitsa ma nuntrallery a ma acid, potero amachotsa zosayera.

Elotions: Pomaliza, acid oyeretsedwa a nucleated acid ali mu buffer yoyenera, okonzeka kugwiritsa ntchito ma pcr, zotupa, kapena kudzula.

N 'chifukwa Chiyani Muyenera Kugwiritsa Ntchito Nyukiti ya Nucleic Acid?

Kuchita bwino: Njira zachikhalidwe zam'madzi a acidic zowonjezera ndi nthawi yambiri komanso nthawi yambiri. Ma nucleic acid m'zigawo za manyuzikidwe amasinthasintha njira ndipo amatha kumaliza kuchotsa mkati mwa ola limodzi.

Kusinthanitsa: Protocols yokhazikika yomwe imaperekedwa ndi izi kuti zitsimikizire kubereka komanso kudalirika kwa zotsatira zake. Izi ndizofunikira pakuyesa komwe kulondola kuli kovuta, monga kafukufuku wazachipatala kapena kafukufuku.

Kusiyanitsa: Ma Kits ambiri adapangidwa kuti azigwira mitundu mitundu, ndikuwapangitsa kukhala oyenera mapulogalamu osiyanasiyana. Kaya mukugwira ntchito ndi zitsanzo za anthu, minofu yazomera, kapena zikhalidwe zazing'ono, pali mtundu kuti ugwirizane ndi zosowa zanu.

Makampani osuta: ambiri a acid zigawo za nyukiliya zimachokera ndi malangizo atsatanetsatane ndipo adapangidwa kuti akhale osavuta kugwiritsa ntchito, ngakhale kwa iwo omwe sangakhale ndi zochitika zambiri zapamwamba. Izi zili ndi demokalase yofikira njira zamatoologral bleology, kulola ofufuza ambiri kuti atenge nawo gawo kafukufuku wa genetics.

Kugwiritsa ntchito exvactic acid

Acid a ma acid omwe adapezeka kuchokera ku Kits awa akhoza kukhala maziko a mapulogalamu angapo:

NJIRA ZOSAVUTA: Kumvetsetsa Cene, mawu ndi malamulo.
Kuzindikira zamankhwala: Kuzindikira matenda a chibadwa, matenda opatsirana ndi khansa.
Sayansi: Kusanthula zitsanzo za DNA pazakufufuza.
Zaulimi Biotechnology: Kukula kwa zolengedwa zosinthidwa zosinthidwa (GMOS) kuti muwonjezere zokolola.
Pomaliza
Nucleic acid pochotsa ma kitsndi zida zosafunikira mu biology yamakono, kulola ofufuza kuti atsegule zinsinsi za moyo ku masele a molecular. Kuchita bwino kwawo, kusasinthika kwawo, komanso kusinthasintha kwasintha kukhala kwa kafukufuku ndi kuwunika, kumapangitsa kuti zikhale zosavuta kudziwa zovuta za DNA ndi RNA. Monga ukadaulo ukupitilirabe, titha kuyembekezera kuti chipalechi chisinthe, kutsegula zitseko zatsopano zopezeka ndi sayansi komanso zatsopano. Kaya ndinu ofufuza mwatsatanetsatane kapena watsopano kuthengo, kuwononga muyeso wa acidic acid omwe amatha kusintha ntchito yanu ndikuthandizira kuti ntchito yanu idziwe zambiri.