Kusiyana kwachuma komanso kufunikira kwa mbale zakuya kwambiri mu labotale yamakono

M'dziko lonse lapansi la kafukufuku wa asayansi ndi kuyesa, zida ndi zida zomwe zimagwiritsidwa ntchito mu labotaries zimachita gawo lofunikira popambana ma projekiti osiyanasiyana. Chida chimodzi chofunikira choterechi ndi mbale yakuya. Mapulogalamu apaderawa ayamba kukhala oyenera, makamaka m'minda monga mabizinesi a sayansi yazomwe, sayansi, ndi kupezeka kwa mankhwala. Mu blog ino, tionanso kuti tisamakayi komanso kufunika kwa mbale zakuya, zomwe amagwiritsa ntchito, komanso zabwino zomwe amabweretsa kwa ofufuza.

Kodi mbale yakuya ndi iti?

A mbale yozamandi malo otetemera ndi zitsime zingapo, aliyense amapangidwa kuti azigwiritsa ntchito zamadzimadzi ambiri kuposa maikolofoni wamba. Mbale zakuya kwambiri nthawi zambiri zimapangidwa ndi pulasitiki zapamwamba ndikubwera mumitundu yosiyanasiyana yokhala ndi ma cartics abwino kuyambira 1 ml mpaka 50 ml kapena kupitilira. Mbale izi zimapangidwa kuti zilolere zosungira bwino, kusakaniza, ndi kusanthula, kuwapangitsa kukhala chida chofunikira m'makonzedwe ambiri a labotale.

Kugwiritsa ntchito mbale yayikulu

Mbale zakuya kwambiri zimakhala ndi mapulogalamu angapo, kuphatikiza koma osangokhala:

1. Kusungira kwachitsanzo: Ofufuza nthawi zambiri amagwiritsa ntchito mbale zolimba posungiramo nthawi yayitali zitsanzo za kulera monga DNA, RNA, mapuloteni, ndi zikhalidwe zam'manja. Kukula bwino bwino bwino, the bambani lotetezeka kungakhale kusungidwa popanda chiopsezo cha kusintha kapena kuipitsidwa.
2. Kuwunikira kwambiri: Popezeka ndi mankhwala osokoneza bongo komanso chitukuko, mbale zakuya ndizofunikira pakuwunika kwa-kwezani (HTS). Amathandizira ofufuza kuti ayesere zinthu zikwiziri nthawi imodzi, ndikuwathamangitsa kwambiri chizindikiritso cha omwe angagwiritse ntchito mankhwala.
3. PCR ndi QPCR: Mbale zakuya nthawi zambiri amagwiritsidwa ntchito pochita ma polymeatiase (PCR) ndi kuchuluka kwa PCR (QPCR). Adapangidwa kuti athetse njinga yamatenthedwe ndikuchepetsa chiopsezo cha kuipitsidwa pakati pa zitsanzo.
4. Protein crystallization: Pamasewera a sayansi, mbale zakuya kwambiri zimagwiritsidwa ntchito poyesa ma protein. Mabowo akuluakulu amapereka malo okwana kukula kwa kristalo, omwe ndi ofunikira pa maphunziro a X-ray Crystallography.
5. Chikhalidwe cha foni: Mbale zakuya zimagwiritsidwanso ntchito maselo achikhalidwe m'malo olamulidwa. Mapangidwe awo amalola mizere yambiri ya maselo kuti ikhale yodziwika bwino nthawi yomweyo, kuthandizira maphunziro ndi zoyeserera zofananira.

Ubwino wogwiritsa ntchito mbale zakuya

Kugwiritsa ntchito mbale zozama kumapereka zabwino zambiri zomwe zimakulitsa kuchita bwino komanso kulondola kwa labotale yanu:

1. Kuchulukana Kukula: Ubwino waukulu wa ma mbale akulu ndi kuthekera kwawo kogwiritsa ntchito madzi ambiri, omwe ndi othandiza kwambiri kuyesa komwe kumafuna zitsanzo zambiri.
2. Kuchepetsa chiopsezo cha kuipitsidwa: Kapangidwe ka mbale--bwino kwambiri kumachepetsa chiopsezo chodzaipitsidwa pakati pa zitsanzo ndikuwonetsetsa kukhulupirika kwa zoyeserera.
3. Kugwirizana ndi Kupanga Kwachangu: Mbale zambiri zakuya kwambiri zimagwirizana ndi makina ogwiritsira ntchito okha madzi, zomwe zimapangitsa kuti zikhale zowoneka bwino kwambiri ndikuchepetsa kuthekera kwa vuto la munthu.
4. Ntchito Zosiyanasiyana: Monga tatchula kale, mbale zakuya zitha kugwiritsidwa ntchito m'njira zosiyanasiyana, zimapangitsa kuti azichita chida chosindikizira zosiyanasiyana.
5. Mtengo wothandiza: Pogwiritsa ntchito zitsanzo zingapo nthawi imodzi, mbale zakuya kwambiri zimatha kusunga nthawi ndi zinthu zina, pamapeto pake zimasunga ndalama zogwirira ntchito labotale.

Pomaliza

Pomaliza,mbale zozamandi gawo lofunikira la ma labotale amakono. Kugwiritsa ntchito zinthu zawo zosiyanasiyana, komanso kusiyanasiyana kwa magetsi kumawapangitsa kuti azichita chida chamtengo wapatali m'minda yambiri. Monga kafukufuku wa sayansi akupitilizabe, kufunikira kwa mbale zakuya kumangokula, kumatula njira zopezera zatsopano ndi zinthu zatsopano. Kaya mukutenga nawo mbali kwa mankhwala osokoneza bongo, biology suclogy, kapena kulanga kwina konse, kuwononga mbale zapamwamba kwambiri kumatha kukulitsa luso lanu.