Upangiri Wofunikira ku Machubu a 8-Strip PCR: Kusintha Mayendedwe Anu a Labu

Pankhani ya biology ya mamolekyulu, kulondola ndi kuchita bwino ndikofunikira kwambiri. Chida chimodzi chomwe chimapangitsa kuti ma labotale aziyenda bwino ndi chubu cha 8-plex PCR. Machubu otsogolawa adapangidwa kuti achepetse njira ya polymerase chain reaction (PCR), kulola ofufuza kuchita zoyeserera mosavuta komanso molondola. Mubulogu iyi, tiwona zaubwino wa machubu a PCR a 8-plex, zochitika zamagwiritsidwe ntchito, ndi malangizo amomwe angakulitsire kuthekera kwawo mu labotale.

Kodi machubu a PCR a mizere 8 ndi chiyani?

8-mizere PCR machubuamapangidwa ndi machubu asanu ndi atatu osiyana a PCR olumikizidwa mndandanda kuti apange chubu chamzere. Kapangidwe kameneka kamalola kuti zitsanzo zambiri ziwonjezeke nthawi imodzi, zomwe zimapangitsa kuti zikhale zoyenera kwambiri pazoyeserera zapamwamba kwambiri. Aliyense PCR chubu mu Mzere chubu akhoza kukhala yeniyeni buku la anachita osakaniza, kawirikawiri 0,1 ml kuti 0,2 ml, amene ali oyenera zosiyanasiyana PCR ntchito.

Ubwino wogwiritsa ntchito machubu a 8-strip PCR

1. Konzani bwino: Ubwino umodzi wofunikira wogwiritsa ntchito machubu a PCR a mizere 8 ndikupulumutsa nthawi yokonzekera. M'malo mogwira machubu a PCR pawokha, ofufuza amatha kutsitsa zitsanzo zingapo nthawi imodzi, kuchepetsa chiopsezo cha kuipitsidwa ndi zolakwika za anthu.
2. Zachuma komanso zogwira mtima: Pogwiritsa ntchito mizere yoyesera, ma laboratories amatha kuchepetsa kuchuluka kwa zinthu zomwe zimagwiritsidwa ntchito poyesera. Izi sizingochepetsa ndalama zokha, komanso zimachepetsa mphamvu ya mapulasitiki otayika pa chilengedwe.
3. Kutsatiridwa bwino kwachitsanzo: Machubu ambiri a 8-strip PCR amabwera ndi malo odziwika bwino, zomwe zimapangitsa ofufuza kuti azindikire zitsanzo mosavuta. Izi ndizofunikira pamayesero pomwe kutsata kolondola ndikofunikira kuti zitsimikizire kuti zitha kupangidwanso.
4. Zodzichitira n'zogwirizana: Pamene ma laboratories akuchulukirachulukira kutengera ukadaulo wodzipangira okha, mapangidwe a chubu cha PCR cha 8-mizere imagwirizananso ndi makina opangira. Kugwirizana kumeneku kumawonjezera kupititsa patsogolo ndikuthandizira mapangidwe ovuta kwambiri oyesera.
5. Kusinthasintha: Machubu a PCR a 8-strip angagwiritsidwe ntchito pazinthu zosiyanasiyana, kuphatikizapo kuchuluka kwa PCR (qPCR), reverse transcription PCR (RT-PCR), ndi genotyping. Kusinthasintha kwake kumapangitsa kukhala chida chofunikira kwa ma labotale ambiri a biology.

Kugwiritsa ntchito chubu cha 8-strip PCR

Kugwiritsa ntchito machubu a 8-strip PCR ndi ambiri komanso osiyanasiyana. Amagwiritsidwa ntchito kwambiri:

• Matenda a matenda: M'ma laboratories azachipatala, machubu a 8-strip PCR angagwiritsidwe ntchito kuti azindikire matenda opatsirana, matenda obadwa nawo, ndi zolembera za khansa.
• Kafukufuku ndi chitukuko: Pakafukufuku wamaphunziro ndi mafakitale, machubu awa ndi ofunikira pakufufuza za majini, kupanga katemera, ndi ntchito zina zamamolekyulu a biology.
• Sayansi yazamalamulo: Kutha kukulitsa DNA kuchokera ku zitsanzo zazing'ono kumapangitsa kuti machubu a PCR a mizere 8 akhale ofunikira pakufufuza kwazamalamulo, komwe umboni uliwonse umawerengera.

Malangizo pakukulitsa kugwiritsa ntchito machubu a PCR a 8-strip

1. Konzani zochitika: Onetsetsani kuti zinthu za PCR zakonzedwa kuti muyesere. Izi zikuphatikizapo kusintha kutentha kwa annealing, nthawi yowonjezera, ndi ndende ya enzyme.
2. Gwiritsani ntchito ma reagents apamwamba: Kupambana kwa PCR kumadalira kwambiri mtundu wa ma reagents omwe amagwiritsidwa ntchito. Pokhapokha posankha DNA polymerase yapamwamba, zoyambira, ndi ma buffers zitha kupezeka zotsatira zodalirika.
3. Pitirizani kusabereka: Popewa kuipitsidwa, nthawi zonse gwiritsani ntchito njira ya aseptic pogwira machubu a PCR a mizere 8. Izi zikuphatikizapo kuvala magolovesi, kugwira ntchito pamalo aukhondo, komanso kupewa kuipitsidwa pakati pa zitsanzo.
4. Kusungirako koyenera: Sungani machubu a PCR a mizere 8 osagwiritsidwa ntchito pamalo ozizira, owuma kuti asunge kukhulupirika kwawo. Tsatirani malangizo osungira omwe amapanga.

Pomaliza

8-mizere PCR machubundi ukadaulo wosokoneza pankhani ya biology ya mamolekyulu, okhala ndi maubwino ambiri omwe angapangitse kuti ma labotale akhale olondola komanso olondola. Pomvetsetsa ubwino ndi ntchito zake, ofufuza angagwiritse ntchito zidazi kuti apititse patsogolo kayendetsedwe ka ntchito ndikupeza zotsatira zodalirika. Kaya mukufufuza zachipatala, kafukufuku wasayansi kapena kusanthula kwazamalamulo, kuphatikiza machubu a PCR a mizere 8 muzochita za labotale kungapangitse kuti ntchito yanu ikhale yabwino. Landirani tsogolo la PCR ndikuwona zoyeserera zanu zikuyenda bwino ndi njira yatsopanoyi!