Kusintha kafukufuku: Dongosolo lenileni la PCR

M'dziko la biology ya ma biology ndi ma genetics, makina enieni a PC ali atatulukira ngati masewera, kusintha njira yofufuza ndikusanthula ma acid a ma acid. Tekinolojeni yodula iyi yatulutsa njira yopita patsogolo kwambiri m'minda monga matenda azachipatala, kuwunika kwachilengedwe, ndi mankhwala osokoneza bongo. Mu positi ya blog iyi, tidzayang'anizana ndi zovuta za PCR Systems, ikufufuza kuthekera, kugwiritsa ntchito, komanso momwe zimakhudzira kafukufuku wasayansi.

Kuzindikira ukadaulo weniweni PC

PCR yeniyeni ya nthawi yeniyeni, yomwe imadziwikanso kuti PCR (QPCR), ndi njira yamphamvu kwambiri ya suboglar yomwe imagwiritsidwa ntchito ndikuwonjezera nthawi yomweyo kuchuluka kwa molekyulu ya DNA. Mosiyana ndi pcr yachikhalidwe, yomwe imapereka gawo loyenerera la DNA, PCR yeniyeni PCr limalola kuwunika kosalekeza kwa njira yopitilira munthawi yeniyeni. Izi zimatheka pogwiritsa ntchito utoto wa fluoressestscent kapena zojambula zomwe zimapangitsa kuti DNA Kukwezedwa kumadutsa. ADongosolo lenileni la PCRili ndi zida zapadera ndi mapulogalamu apadera omwe amathandizira muyeso woyenera ndi kusanthula kwa deta ya Empnication, kupereka ofufuza ndi zotsatira zolondola komanso zodalirika.

Mapulogalamu mu matenda azachipatala

Chimodzi mwazinthu zofunikira kwambiri pa dongosolo lenileni la PCR ili m'munda wa matenda azachipatala. Tekinolojeyi yakhala yothandiza pakupezeka ndi kuchuluka kwa tizilombo toyambitsa matenda monga ma virus, mabakiteriya, ndi bowa. Munthawi ya matenda opatsirana, PCR yeniyeni ya PCR imathandizira kuzindikiritsa kwapang'onopang'ono kwa othandizira, kulola kuzindikira koyambirira komanso kulowererapo panthawi yake. Kuphatikiza apo, PCR yeniyeni yakhala ikuyang'ana m'kuwunika kwa mitundu yosiyanasiyana ya matenda osiyanasiyana, ndikuthandizira kuzindikira zamagetsi moleklaula ndi kupita patsogolo.

Kuwunika zachilengedwe ndi kafukufuku

Dongosolo lenileni la PCR lomwe limapezekanso limagwiritsidwanso ntchito potengera zachilengedwe kuwunika ndi kufufuza. Kuyambira kuwunika microbial kusiyanasiyana mu dothi ndi zitsanzo zamadzi kuti mutsatire kufalikira kwa zinthu zosinthika zosinthidwa, PCr yeniyeni imapereka chida chosintha ma acid a zinyama. Kuphatikiza apo, ukadaulo uwu wakhala wosagwirizana ndi zodetsa zachilengedwe komanso zodetsa, zomwe zimathandizira kuyesetsa kuteteza zachilengedwe komanso thanzi la anthu.

Kukhudzidwa pamankhwala ndi kafukufuku

Munthawi yakutha kwa mankhwala ndi kafukufuku, dongosolo lenileni la PCR lakhala likugwira ntchito yofunika kwambiri pofufuza mankhwala osokoneza bongo, poizoni, ndi pharmacogenomics. Mwakuthandizira kuyerekezera kwa mitundu ya mitundu ndi DNA / RNA / RNA / PCR ya nthawi yeniyeni imathandizira kuwunika kwa kusintha kwa mankhwala osokoneza bongo. Izi zili ndi tanthauzo la mankhwala, monganso PCR yeniyeni imatha kuthandiza kuzindikiritsa ma genetic omwe amakopa mayankho amodzi m'mankhwala apadera, poteteza njira zochizira ndikuwongolera zotsatira za wodwala.

Ziyembekezero zamtsogolo ndi kupita patsogolo

Monga ukadaulo ukupitiliza kusinthika, dongosolo lenileni la PCR limakhala ndi chidwi chopita patsogolo, kulimbikitsa luso lake ndikukulitsa ntchito zake. Kuyesayesa kosaka kafukufuku kumayang'ana pakusintha kwa chidwi, kuphatikiza kuchuluka kwa mapangidwe a PC yeniyeni, ndi cholinga chopangitsa kuti ukadaulo uzipezeka komanso wogwiritsa ntchito. Kuphatikiza apo, kuphatikiza kwa PCR yeniyeni ndi njira zina zowunikira, monga njira yotsatira, imalonjeza kuti itsegule madera atsopano omwe akuwunika kwa mitundu ya genomic.

Pomaliza,Dongosolo lenileni la PCRImayimira ngati mwala wapangodya wamakono ndipo wasiya chizindikiro chosafufuzira. Kutha kwake kupereka mwachangu mwachangu, molondola, komanso kuchuluka kwa zigawo za nuclec. Monga ofufuza akupitiriza kukakamira mphamvu ya PC yeniyeni, titha kuyembekezera zotsitsimula zina zomwe zidzapangitse tsogolo la biotechnology ndi mankhwala.