Sinthani PCR: FrestCycler Wormler

M'munda wa biology wazomwe,Mafuta ozungulira ndi chida chofunikira kwambiri mu ma polymeatiction Association (PCR). Monga ofufuza ndi ma labotorees amatsatira kuchita bwino komanso molondola, othamanga atha kukhala masewera. Ndiukadaulo wake wodulidwa ndi magwiridwe antchito apamwamba, othamanga akukhazikitsa muyeso watsopano wamafuta.

Wothamanga amathandizidwa ndi zinthu zapamwamba kwambiri zochokera ku Marlow, USA. Izi zinthuzi zimapangidwa mosamala kuti zizitentha kutentha, ndi kutentha kwa kutentha kwa 6 ° C / s. Kuthamanga kofulumira kumeneku kumachepetsa nthawi yomwe PCR iliyonse imathandizira kuzungulira kwa PCR, kulola ofufuza kuti ayesere kumaliza mwachangu popanda kusokonekera.

Chimodzi mwazinthu zoyeserera zam'madzi ndi kuwerengera kozungulira, kuzungulira kopitilira 100 miliyoni. Kukhazikika kumeneku kumatsimikizira kuti ma labu amatha kugwiritsa ntchito zosewerera kwa nthawi yayitali, ndikupangitsa kuti ikhale yotsika mtengo pazogulitsa zilizonse. Moyo wautali umatanthawuza kuti ofufuza angayang'ane zoyeserera zawo osadandaula za kulephera kwa zida kapena kufunika kosinthasintha.

Kulondola kutentha kumafuna ku PCR, ndipo othamanga amapitilira pankhaniyi. Kugwiritsa ntchito ukadaulo wapamwamba kwambiri ndi ukadaulo wozizira, kuphatikiza ndi pid (molingana ndi ma pid (molingana-ophatikizira) ophatikiza) Izi ndizofunikira kwambiri kukwaniritsa zotsatira zodalirika komanso zobereka, zomwe ndizofunikira pakufufuza kwa asayansi.

Kufanana pamatutu onse ndi gawo linanso lovuta kwambiri pa njinga yamatenthedwe, ndipo zosefukira sizikhumudwitsa. Wothamanga amapangidwa kuti awonetsetse mawonekedwe osasinthika bwino pamavuto lililonse lililonse, kuchepetsa chiopsezo cha kusiyanasiyana zotsatira za PCR zotsatira. Kufananaku ndikofunikira kwambiri pakugwira ntchito ndi zitsanzo zomwe zimafuna kutsirizidwa kotsimikizika, monga kumathandizira kuti zochita zonse zichitike motere.

Kuphatikiza apo, othamanga amagwira ntchito mwakachetechete, ndikupanga zabwino kwa zola zomwe zimafuna malo okhazikika antchito. Izi zimathandizira ofufuza kuti aziyeserera zoyesa popanda kusokonezedwa ndi phokoso lamakina, ndikupanga mkhalidwe wokhazikika komanso wofunikira kwambiri.

Kuphatikiza pazifukwa zake zaukadaulo, othamanga adapangidwa kuti azigwiritsa ntchito mochenjera m'maganizo. Njira zake zolowerera komanso zosavuta za mapulogalamu zimapangitsa kuti zikhale zosavuta kwa ofufuza ndi ma novice omwe amagwiritsa ntchito. Kutha kusintha ma protocols osasinthika kumawonjezera luso la ogwiritsa ntchito, kulola asayansi kuyang'ana kafukufuku wawo m'malo mogwiritsa ntchito makina ovuta.

Mwachidule, othamangaThermal cyclerimayimira kupita patsogolo kwakukulu mu ukadaulo wa PCR. Ndi zinthu zake zapamwamba za petiriry, zotayika mwachangu, zotchinga bwino, komanso kuwongolera kutentha, kumathandiza kuti pakhale magwiridwe osayerekezerera kwa mabizinesi a salecular. Kuphatikizika kwa kulondola, kufanana, ndi phokoso lotsika-phokoso kumapangitsa kuti chitsimikizo chofunikira pazinthu zilizonse zofunika kuchita kuti apeze zotsatira zabwino kwambiri. Monga kufunikira kwa PCr mwachangu komanso wodalirika kumapitilirabe, wofulumirayo amayenda ngati mtsogoleri yemwe ali m'munda, akuthandizira ofufuza kukakankha malire a kupezeka kwa sayansi.