Protocol yodziwikiratu zakuchokera nyama za Bigfish

Vuto la chitetezo cha chakudya likukulirakulirakulira. Pamene kusiyana kwa mtengo wa nyama kukukulirakulira pang’onopang’ono, chochitika cha “kupachika mutu wa nkhosa ndi kugulitsa nyama ya galu” kumachitika kawirikawiri. Kuganiziridwa kuti ndi zachinyengo komanso kuphwanya ufulu ndi zofuna za ogula, kumachepetsa mbiri ya anthu pa nkhani ya chitetezo cha chakudya, zomwe zimapangitsa kuti anthu azivutika. Pofuna kuonetsetsa kuti chakudya chili chotetezeka komanso kuti pakhale chitetezo choweta ziweto m'dziko lathu, miyezo ndi njira zowunikira ndizofunikira kwambiri.

Ndi luso lopitilirabe komanso kulimbikira kwa ofufuza, Bigfish yapanga payokha zida zodziwira zotengedwa ndi nyama, zomwe zimapereka mayankho apamwamba komanso ofulumira kwa makasitomala athu! Timamvanso kuti ndi mwayi waukulu kuthandiza makasitomala athu kuthetsa mavuto awo.
Dzina lazogulitsa: Zida zodziwira zoyambira zanyama (nkhumba, nkhuku, kavalo, ng'ombe, nkhosa)
Kukhudzika kwakukulu: malire ozindikira osachepera 0.1%
Kukhazikika kwapamwamba: kuzindikiritsa zolondola zamitundu yonse ya "nyama yeniyeni ndi yabodza", palibe kuwoloka
1, Zitsanzo processing
Zitsanzozo zimatsukidwa kawiri katatu ndi 70% ethanol ndi madzi osungunuka kawiri, amasonkhanitsidwa mu machubu oyera a 50 ml centrifuge kapena matumba oyera osindikizidwa ndikusungidwa oundana pa -20 ° C. Zitsanzozo zinagawidwa m'magawo atatu ofanana, kuphatikizapo chitsanzo choyesedwa, chitsanzo choyesedwanso ndi chitsanzo chosungidwa.
2. Kutulutsa kwa nucleic acid
Zitsanzo za minofu zimawumitsidwa ndikuphwanyidwa bwino kapena kuwonjezeredwa ku nayitrogeni wamadzimadzi, kenako amathira mumatope ndi pestle, ndipo DNA yamtundu wa nyama imachotsedwa pogwiritsa ntchito njira yokhayo.nucleic acid extractor + Magpure Animal Tissue Genomic DNA Purification Kit.

(Laboratory m'zigawo seti)

3. Mayeso okulitsa
Kuyesa kokulitsa kumachitika pogwiritsa ntchito makina a Bigfish sequential real-time quantitative fluorescence PCR analyzer + zida zodziwikiratu zochokera ku nyama kuti adziwe bwino ngati nyamayo yaipitsidwa malinga ndi zotsatira zoyipa, kuteteza bwino ufulu wa ogula komanso chitetezo cha chakudya.

Zitsanzo: Zida Zoyesera Zoyambira Zanyama (Nkhosa)