Magwiridwe a ma nyukini anayi a acine acid acids kuti azindikire SAS-Cov-2 ku Ethiopia

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Kuyambira mu 2019 Coronavirus Matenda (Covid-19), ambiri amalonda acid amayesedwa (Naut) adapangidwa padziko lonse lapansi ndipo akhala akatswiri wamba. Ngakhale mayesero angapo adapangidwa mwachangu ndikugwiritsa ntchito mayeso a labotale, mayeserowa sanawoneke m'malo osiyanasiyana. Chifukwa chake, kafukufukuyu anafuna kuyesa magwiridwe antchito a Abbott-cov-2, daan gene, bgi, ndi zonunkhira bwino pogwiritsa ntchito muyezo wophatikizira (CRS). Phunziroli lidachitika ku Indiiopian Consitain Institute Institute (Efi) kuyambira 1 mpaka 30 Disembala 2020. 164 Sasopharyngeal Samops adachotsedwa pogwiritsa ntchito QNAM SAMP PRID. Za fanizo 164, 59.1% anali olimbikitsa ndipo 40.9% anali oyipa kwa ma crs. Sansare Biotech Issotivity inali yotsika kwambiri poyerekeza ndi CRS (P <0.05). Sansare Biotech Issotivity inali yotsika kwambiri poyerekeza ndi CRS (P <0.05). Ntchafuитттез Zotsatira zabwino za Sansate Biotech zinali zotsika kwambiri poyerekeza ndi CRS (P <0.05).与 CRS 相比, Sansare Biotech 的 (P <0.05).与 CRS 相比, Sansare Biotech 的 (P <0.05). У ovala biotech зыло меньатотототовт положв положв положв полож Sansare Biotech anali ndi zotsatira zabwino zambiri poyerekeza ndi Cer (P <0.05).Chigwirizano chonse cha kusanthula kwake chinali 96.3-100% poyerekeza ndi ma Cer. Kuphatikiza pa kuchuluka kochepa kwa otumphuka biotech kukalowa, magwiridwe akewa anali ofanana kufananizidwa. Mwakutero, wonyezimira biotech [kafukufuku wokha (Ruo)] Assual] amafunika kutsimikizika kowonjezera pakugwiritsa ntchito ku Ethiopia. Pomaliza, kafukufuku wowonjezera ayenera kuona kuti ndiwakuwunikiranso zonena za wopanga zoyenera.
Kuyesa kwa labotale ndi gawo la World Health Organisation (omwe) dongosolo la mapulani a Coronavirus Matenda 2019 (Covid-19) kukonzekera ndi kuyankha (sprp). Yemwe amalangiza kuti mayiko amafunikira kuti azitha kusintha zinthu zakale kusinthana, kasamalidwe koyenera, kukhala maso komanso kuyankha mwachangu kuthana ndi mavuto azaumoyo. Izi zikusonyeza kuti gawo la labotale ndi kiyi yodziwika ndi matenda ndi epidemiology ya omwe akubwera ndi kupatsira matenda omwe amabwera ndikuwongolera kufalikira kwawo.
Kuzindikira kwa Covid-19 kumafuna chidziwitso cha miliri komanso zamankhwala, zizindikiro zanu, ndi zizindikiro, ndi radiographic ndi labotatophy deta2. Popeza kuchuluka kwa Covird kunanenedwa ku Wuhan, China, zambiri zamalonda acid kumayesedwa (Nauts) zakonzedwa padziko lonse lapansi. Kulemba kwenikweni kwa nthawi yosinthira kwa polymetriose (Rrt-PCR) yagwiritsidwa ntchito ngati njira yosinthira kwa laboratory ya Laborator Hictiomer Syndrome 2 (SAV-2) 3) 3) Matenda. Kuzindikira kwa SARS-Cov-2 kumakhazikika ku n (ma proterin gene), e (envelope quargen gene), enlolp protein gene), rdrp (kuwerenga RNA / B). Chigawo) dera chodziwika kuchokera ku gerome. Amawerengedwa kuti ndi malo osungidwa omwe amapezeka mu virus a virus omwe akuzindikira kachilomboka. Mwa mitundu iyi, ma gene a ntrrp ndi E
Kuchita kwa ma PCR kumasiyananso kutengera zinthu zosiyanasiyana monga: m'zigawo zopanga ma reagents, zowunikira, njira yochotsera, mtundu wina wa makina a PCR ndi zida zina. Pofika pa Epulo 2020, zida zopitilira 48 zosokoneza m'maiko 9 zomwe zalandira zovomerezeka zadzidzidzi (Eua) kwa diavid-196. Ku Ethiopia, zoseweretsa zopitilira 14 zenizeni zimagwiritsidwa ntchito pakuzindikira kwa PCR ya SARS-Cov-2 mawebusayiti a Health Ecuant Maphunziro, kuphatikizapo Abi 7500, Roche 48000 ndi Stuche 48000 ndi Stuthe7. Kuphatikiza apo, ma kits osiyanasiyana a PCR amapezeka, monga daan gene, mayeso a Abbott - mayeso a 2, mayeso a Sacerech, ndi SAV-2 BGI. Ngakhale Rrt-PCR ndiovuta kwambiri, odwala ena omwe ali ndi covid-19 zotsatira za makope osakwanira chifukwa cha makope a Virtulecleic acid (RNA) m'Chitsanzo choyenera, Kuyendetsa machitidwe ndi zochita za anthu. Kuphatikiza apo, zitsanzo kapena kuwongolera zolakwika za ma C2 Chifukwa chake, zikuonekeratu kuti mayeso a PCR angazindikire onyamula zidutswa za gene, chifukwa sangasiyanitse pakati pa majini a virus, motero mayesowo amatha kungozindikira zonyamulira zokhazokha osati zodekha koma osati odekha10. Chifukwa chake, ndikofunikira kuwunika magwiridwe ozindikira pogwiritsa ntchito njira zofananira munthawi yathu. Ngakhale kuti ma Naoni ambiri amapezeka ku Indiite Inshuwaransi ku Inshuwaransi (Efi) ndi dziko lonselo, kuwunika kwamphamvu kwamphamvu komwe kwanenedwapo. Chifukwa chake, kafukufukuyu anafuna kuyesa njira yofananira yofananira kwa mapiri omwe amapezeka pakudziwika kwa SARS-Cov-2 ndi Rrt-2 omwe amagwiritsa ntchito zoyerekeza zamankhwala.
Ophunzira okwana 164 omwe ali ndi Covil yemwe akuwoneka kuti ali ndi Covid wazaka 19 adaphatikizidwa mu kafukufukuyu. Zitsanzo zambiri zinali zochokera m'malo othandizira (118/164 = 72%), pomwe ophunzira 46 (28%) adachokera m'malo osalandira chithandizo. Pakati pa ophunzira sanathandizidwe pakati, 15 (9.1%) adakayikira milandu ndi 31 (18.9%) adalumikizana ndi milandu yotsimikizika. Makumi asanu ndi anayi mphambu makumi asanu ndi anayi mphambu atatu (56.7%) ndi amuna, ndipo tanthauzo (± 11.10 (± 11.12).
Pa phunziroli, mitengo yabwino ndi yoipa yoyesedwa kanayi kwa Covid-19 idatsimikizika. Chifukwa chake, mitengo yabwino ya Abbott SARS-Cov-2 Garch, Daan Genes, Scov-2%, 58.5% ndi 55.5% motsatana. Malingaliro abwino komanso osalimbikitsa (Cers) anali 97 (59.1%) ndi 67 (40.9%), motsatana (tebulo 1). Mu phunziroli, ma Cer anali ophatikizidwa ndi "ufulu" uliwonse, womwe umachokera ku zotsatira zinayi kapena zoyeserera ziwiri kapena zingapo zoyeserera zomwezo zidawerengedwa zabwino kapena zoipa.
Mu kafukufukuyu, tinapeza pangano loipa (NPA) la 100% (95% CI 94.6-100) chifukwa cha kusanthula konse poyerekeza ndi ma CR. Kusanthula kwa Gootechnology kuwonetsera PPA ya 93.8% (95% CI 87.2-97.1-97.1-97.1) ndi Daan Genels anali ndi mgwirizano wa 99.4% (95% CI 96.6). Mosiyana ndi izi, mgwirizano wapakati pa SARS-Cov-2 BGI Gawani ndi Sansare Bisch 2018.8% ndi 96.3%, motsatana (tebulo 2).
COHPA yogwirizana ndi mgwirizano wapakati pa CRS ndi Abbott SARS-CAV-2 Agay inali yofanana (k = 1.00). Mofananamo, kappa yotsimikizika ya kappa yomwe idapezeka ndi daan Gene 2019-NCAV, SCOR-2 BGI, ndi kutsuka biotech 20195). Mu kusanthula kumeneku, kuyesa kwa Chi-lalikulu (kuyesa kwa McNemar) kuwonetsa kuti Sansach Biotech 2019-NCAV imakhala yosiyana kwambiri ndi zotsatira za cer (p = 0.031) (tebulo 2).
Monga zikuwonetsera mkuyu.1 peresenti ya mtengo wotsika kwambiri wa CT (<20 ct) ya Abbott SARS-Cov-2 Gantay (<20 cry) anali ndi 25,6%. 1 peresenti ya mtengo wotsika kwambiri wa CT (<20 ct) ya Abbott SARS-Cov-2 Gantay (<20 cry) anali ndi 25,6%.Monga zikuwonetsera mkuyu.1, Процен Наименьшего значVE PRACA и n) Составs гена orf1a / b а а а Ct) Состала 3,2%. 1, kuchuluka kwa mtengo wotsika kwambiri wa CT (<20 ct) kusanthula kwa abbott shers-cov-2 (1) kwa 3.2%.1 所示, abbott SARS-COV-2 检测 (结合 RDRP 和) 最低 基因 基因 基因 基因 基因 基因 基因 基因 基因 基因的 3 3.2%. Monga taonera Chithunzi 1, chotsika kwambiri cha CT chamtengo (<20 ct) cha mayeso a ABBOtt 3.2%. Какарпаказака пока размерере 87,6%, а ННачение ct Нена orf1a / B Monga taonera Chithunzi 1, SAV-CAV-2 (Kuphatikiza v fes (<20 cry) pa 87.6%, Kusanthula kwa NCOV - Kusanthula kwa NCOV Kunawonetsa CT. Проениййй: Составин 50,3 ct) Состави set Kuchuluka kwa mfundo (<20 ct) kunali 50.3%, ndi kuchuluka kwa mfundo zapamwamba za CT (36-40 ct) anali 3.2%.Abbott SARS-Cov-2 B Yolembedwa Yojambulidwa Pamwamba pa 30. Kumbali inayo, pa SGI SAS-Cov-2 Garsay Orf1a / B Gene anali ndi mtengo wapamwamba wa CT (> 36 CT) kuchuluka 4% (mkuyu.). Kumbali inayo, pa SGI SAS-Cov-2 Garsay Orf1a / B Gene anali ndi mtengo wapamwamba wa CT (> 36 CT) kuchuluka 4% (mkuyu.). Д дгороронcы, в анализе BGI Komabe, powunikira kwa BGI SAS-Cov-2 Gener Orf1a / B anali ndi mtengo wapamwamba wa CT (> 36 ct), kuchuluka kwa 4% (mkuyu.).另一方面, 在 BGI SARS-CGI-2 检测中, Orf1a / B 基因具有高 基因具有高 值 值 vt) 的 1) 的 4% (图 1) 的 4). Kumbali inayo, mu sggi stars-cov-2 zowoneka bwino, peresenti ya orf1a / b 1/1 ct) ndi 4% (Chithunzi 1). Д дгоророронcы, в анализе BGI SGI Kumbali inayo, mu SGGI
Mu kafukufukuyu, tinatenga zitsanzo za anthu 164 a NASHEPHARDEAL. Kwa mitundu yonse yamalingaliro, RNNA yodzipatula komanso kuphatikizidwa idachitika pogwiritsa ntchito njira ndi ma kits omwe akuvomerezedwa ndi opanga.
Kafukufukuyu adawonetsa kuti kuyesa kwa Abbott kwa SAS-Cov-2 ali ndi magwiridwe omwe ali ndi ma crs, okhala ndi 100% concordance. Pangano la Kappa la Cohen ndi 1.00, kuwonetsa mgwirizano wonse ndi ma cr. Phunziro lofananalo ndi ku Yunivesite ya Washington ku US adapeza kuti chidwi chonse cha SARBOtt cha SARS-Cames, poyerekeza ndi CDC. . Kafukufuku ku Vienna, Austria adawonetsanso kuti m'zigawo zazikuluzikulu ndi zida zodziwika bwino zomwe zimachepetsa mavuto komanso kupezeka kwamphamvu kwambiri. Chifukwa chake, machesi anzeru a Abbott a SARS-Cov-2 amatha kuphatikizidwa ndi njira yodziwika bwino yomwe imazindikira kuphatikiza majini a cotsa, amapeza zitsanzo zambiri (40 μl).
Zotsatira zathunso zinawonetsanso kuti kuyesa kwa mayeso a daan kunali kofanana ndi ma cr. Izi ndizogwirizana ndi Phunziro14 lomwe lidachitika ku yunivesite ya Ahui ku Huainian, China, ndi zonena za wopanga 100%. Ngakhale panali malipoti a zotsatira zosakhazikika, sample imodzi inali yopanda tanthauzo atathanso kubwereketsa anthu omwewo, koma anali ndi chiyembekezo cha abbott SARS-COV-2 ndi kutsuka biov - 2019. Izi zikusonyeza kuti pakhoza kukhala kusintha kwa mitundu yosiyanasiyana ya mafano. Komabe, mu kafukufuku yemwe anachitika ku China15, zotsatira za gene genes anali osiyana kwambiri (P <0.05) poyerekeza ndi lingaliro lawo la lab. Komabe, mu kafukufuku yemwe anachitika ku China15, zotsatira za gene genes anali osiyana kwambiri (P <0.05) poyerekeza ndi lingaliro lawo la lab. Тем ме менатат аене, в ит лабораторарарараторатого этало эоо ана. Komabe, pophunzira ku China15, kafukufuku wa Daan Gene kunali kosiyana kwambiri (P <0.05) kuchokera kuwunika kwawo kwa labotale., 在中国进行 的 15, 的 的 <0.05)., 在中国进行 的 15, 的 的 的 <0.05 О одооноurce раВнию с ео этоннмлорарарамсто Komabe, mu kafukufuku waku China15, zotsatira za mayeso a Daan zidasiyana kwambiri (P <0.05) poyerekeza kuyesa kwa labotale.Kusiyanaku kungakhale chifukwa cha kuzindikira kwa chiyeso chowerengera kuti azindikire SARS-Cov-2, komanso maphunziro ena atha kukhala ofunikira kuti adziwe zomwe zimayambitsa.
Kuphatikiza apo, kuphunzira kwathu kuwunika momwe amafalitsira a SARS-CGI-2 BGI akukana ndi ma CRA, omwe ali ndi mgwirizano wabwino kwambiri. ). = 98.8%). Mfundo za kappa ya Cohen zidawonetsa mgwirizano wabwino (k = 0.975). Kafukufuku ku Netherlands16 ndi china15 awonetsa zotsatira zosasinthasintha. Mayeso-a SGI-2 BGI ndi mtundu umodzi (Orf1a / B) pogwiritsa ntchito 10 μl Kukweza / Kuzindikira Enje. Ngakhale panali mgwirizano woyenera ndi zotsatira zathu, kusanthula kwaphonya zitsanzo zabwino (1.22%) ya zitsanzo zonse. Izi zitha kukhala ndi malingaliro akulu azachipatala operekera magwiridwe antchito onse onse odwala ndi ammudzi.
Kusanthula kwina komwe kumachitika mu phunziroli kunali koteketse biotech NCEV - 2019 RT-PCR (RUO); Maperesenti osewera anali 96.3%. Mphamvu yogwirizana idatsimikizikanso ndi mtengo wa kappa, womwe unali 0,925, kuwonetsa mgwirizano wonse ndi ma cr. Apanso, zotsatira zathu zimakhala zofanana ndi kafukufuku yemwe adachitika ku South Sounta University ku Changsha, China, komanso ku dipatimenti ya labozle ya chipatala cha Liuzhou, China walo la Liuzhou, China Ngakhale malongosoledwe omwe ali pamwambawa adalembedwa, mayeso a Chi-Squarge (Macnearmar) adawonetsa kuti zotsatira za Sankech Istach Itay ikufanana ndi CRS (P <0.005). Ngakhale malongosoledwe omwe ali pamwambawa adalembedwa, mayeso a Chi-Squarge (Macnearmar) adawonetsa kuti zotsatira za Sankech Istach Itay ikufanana ndi CRS (P <0.005). Несмотря на Тто, Тылззикви - кррй (критериме рА )лззарениса 0,005). Ngakhale mgwirizano wabwino wolembedwa pamwambapa unajambulidwa, mayeso a chi-chinenerochi (McNemar) zotsatira za Sankech Biotech Istherch Bitch Courth Coursoss poyerekeza ndi ma CR (P <0.005).上 上述良好 上述良好 上述良好 上述良好 上述良好 上述良好 述良好 的 述良好, 但卡方检验 (MacNARARY 检验) 表明, Sanan 结果与 结果与 结果与 crs 相比具有统计学显着差异 (p <0.005).尽管 了 了 了 了 一致性 一致性 一致性, 一致性 一致性 (a Macnemar 检验 表明 ,: Несмотря на ОтоечCе выотвтие, Скотвторара) Крр [Phhile la Oklationи куююзнузницзннннннннннннннннннн ((p< 0,005) b Ngakhale mgwirizano wabwino walembedwa pamwambapa, mayeso a chi-chinenerochi (P <0.005) pakati pa kutsuka biotech ndi ma c rs.Zitsanzo zisanu ndi chimodzi (3.66%) adapezeka kuti ndi otsutsa abodza poyerekeza ndi CRS (PHERANI 1); Izi ndizofunikira kwambiri, makamaka kupatsa mphamvu za kufalikira kwa kachilomboka. Zomwe zili pamwambazi zimathandiziranso kuzindikiridwa pang'ono kumeneku.
Pa phunziroli, ma CT adatsimikizika papulatifomu iliyonse ndi mtundu wina, wokhala ndi mtengo wotsika wa CT yemwe adanenedwa mu Abbott SARS-Cov-2. Zotsatirazi zitha kukhala zokhudzana ndi dongosolo la Gebott lophatikizana ndi ma geneticative yolumikizirana kwa SARS-Cov-2. Chifukwa chake, malinga ndi Chithunzi 1, 87.6 Mfundo za CT pamwambapa zidalembedwa. Kuphatikiza pa kugwiritsidwa ntchito kwa ABBOtt ml. 19.
Phunziroli lili ndi zofooka zina: Poyamba, sitikhala ndi njira zowerengera (monga ma virus (monga ma virus ena a labotale kapena mayeso ena). Chachiwiri, zoyerekeza zonse zomwe zinali ku Nasopharyngeal Swab, ngakhale zotsatira zake sizinali kugwirira ntchito zina zotanthauzira, ndipo chachitatu, kukula kwathu kunali kochepa.
Kafukufukuyu adayerekeza momwe magwiridwe anayi a Rrt-PCR a SARS-Cov-2 pogwiritsa ntchito zitsanzo. Malingaliro onse anali ndi mwayi wofanana, ndipo kupatula kutsuka biotech kukalowa. Kupatula apo, kuchuluka kotsika komwe kunadziwika mu sanstech Biotech Assay poyerekeza ndi CRS (P <0.05). Kupatula apo, kuchuluka kotsika komwe kunadziwika mu sanstech Biotech Assay poyerekeza ndi CRS (P <0.05). Креме Того, в Тесте полен по праВ в Kuphatikiza apo, kuyesa kwa biotech bi adawonetsa kuchuluka kwapafupipafupi poyerekeza ndi CRS (P <0.05)., 与 CRS 相比, Sansare Biotech 检测 的 (P <0.05)., 与 CRS 相比, Sansare Biotech 检测 的 (P <0.05). Кроме Того, анал biotech боленатататотото Kuphatikiza apo, kusana kwa biotech kutsutsa kuli ndi mtengo wotsika poyerekeza ndi CRS (P <0.05).Wosakanitsa Biotech NCOV-2019 (Ruo) Kusanthula kwa PPA, NPA ndi Pangano Lapamwamba Kupitilira 93.5% yokhala ndi mphamvu ya cohen kappa ya mtengo wa 0.925. Pomaliza, osamala Biotech Atay (RUO) amafunika kutsimikizika kuti agwiritse ntchito Ethiopia, komanso kufufuza kowonjezera kuyenera kuonedwa kuti akuwunika opanga okha.
Mapangidwe a Phunziro lofananiza anachitika pamatambo anayi azaumoyo ku Dutis Ababa, Eka Chipatala cha Mipingo, Zatsopano Chikumbutso Chikumbutso Chipatala cha Chikumbutso. Zambiri zomwe zasonkhanitsidwa pakati pa Disembala 1 ndi 31, 2020. Malo azachipatala omwe aphunzirawa adasankhidwa mwadala kutengera milandu yawo yayitali komanso kupezeka kwa malo akuluakulu mumzinda. Mofananamo, zida, kuphatikizapo aBI 7500 ndi zida zenizeni za Abbott, adasankhidwa malinga ndi zomwe opanga Nait adasankhidwa pa phunziroli, monga labotale ambiri ku Ethiopia adagwiritsa ntchito osachepera anayi a iwo. Kuyesa kwa Gene, kuyesa kwa Abbott-Cov-2, kuyesa kwa SAV, ndi SAR-2 BGI Kuyeserera pa phunziroli).
Kuyesa kwa SARS-COV-2 kunachitika kuchokera ku 1 mpaka 30 Disembala 2020 pogwiritsa ntchito 3 ml ya ma virus oyendera ma virus (vtm) kuchokera kwa anthu omwe akufuna ku Covid-19 adatchula Efi. Zitsanzo za Nasopharyngeal adasonkhanitsidwa ndi osonkhanitsa ophunzitsidwa ndikutumiza ku Efa ku ma Pasts. Asanachitike nulekisi acid, Sampuli iliyonse imapatsidwa nambala yapadera. Kuchotsera kumachitika kuchokera ku zitsanzo zilizonse nthawi yomweyo pofika pogwiritsa ntchito zolemba komanso zongongoletsera zokha. Chifukwa chake, pochotsera chokha cha Abbott M2000, 1.3 ml (kuphatikiza 0,8 ml potsamira) ya saltured systems (mabbott Moles, USA). ) Gulu la zitsanzo za 96 [92), zowunikira ziwiri zodziwika bwino komanso zowongolera ziwiri (NTC) zidaphatikizidwa munjira yonse (pokonzanso) zozungulira ziwiri za SARS-2 (Eua) munthawi yeniyeni. migodi. Mofananamo, kuchotsera pamanja, kugwiritsa ntchito zitsanzo zomwezo (kuti muchotseko ndi kupezeka). Chifukwa chake, konse, zitsanzo za 140 μl zidali okonzedwa ndikutuluka pogwiritsa ntchito quamm quig kit (qiagen gmbh, qirnan, germany, zowongolera ziwiri ndi zigawo ziwiri) zozungulira. Mavuto opangidwa ndi anthu amakulitsidwa ndikuwunika pogwiritsa ntchito chotupa cha abi 7500 pogwiritsa ntchito SARS-Cov-2 BGI Garsey, Naan Geney Aay.
Kudzipatula Kwawokha ndi Kuyeretsa kwa Sars-Cov-2 Viral Rna Kutsatira mfundo ya maginito pogwiritsa ntchito Abbbott DNA Kukonzekera kwa Reson. Kuzindikira kwa zitsanzo ndi kusungunuka kwa ma virus a ma virus amachitika pogwiritsa ntchito chotchinga cha Guaniidine isothiocyunate kutsutsana ndi mapuloteni ndi vatavate rnase. RNA imalekanitsidwa ndi mapuloteni mwa gawo lolimba la magawo pogwiritsa ntchito silica, mwachitsanzo, mchere wa guidininium ndi alkaline ph wa masiketi a lysis amalimbikitsa kukhazikika kwa asidi (Sio2). Cholinga chakumaso chimachotsa mapuloteni otsala ndi zinyalala kuti apange yankho. Zowonekera za RNA zimangodzipatula ku micraparicy yochokera ku Micrasekir ya Silika pogwiritsa ntchito maginito a zida, maginito a zida10,21. Kumbali inayo, kudzipatula kwa RNA kumachitika ndi njira yolumikizira ya center pogwiritsa ntchito centrifugation m'malo mwa maginito ndi kupatukana kwa micrapartics kuchokera kwa olunjika.
Mayeso owoneka bwino a Abbott-Cov-2 odziwika (Abbott Molecular (Inc.) adachitidwa molingana ndi malangizo a wopanga, omwe adalandira Eua19,22 kuchokera kwa yemwe ndi FDA. Mu protocol iyi, ikani makonda asanachotsere idapangidwa mu madzi osamba pa 56 ° C kwa 30 min. Pambuyo pa virus inctivation, ecleic ya acid imachitika pa chida cha Abbott M2000 kuchokera ku 0,5 ml vtm pogwiritsa ntchito njira yokonzekera ya Abbott M2000. Malinga ndi wopanga. Kukwezedwa ndi kuwunika kunachitika pogwiritsa ntchito chida cha Abbott M2000 RT-PCR, ndipo kuwunika kawiri kwake kunachitika kwa RDRP ndi N In. Rox) ndi Vic P (utoto wolozera) kuti usayang'ane ndi kudziwika ndi zowongolera zamkati, kulola kuzindikiritsa nthawi yomweyo.
Njira yodziwira kuti ili ndi ukadaulo wa RT-PCR. The Orf1a / B ndi N IRS adasankhidwa monga madera omwe amasungidwa ndi tebulo la tergelogy kuti adziwe dera lokulitsa. Pulogalamu inayake ndi fluorescenty reates (n RA KI KILARS yolembedwa ndi banja, Orf1a / B Puloses olembedwa ndi Vic) adapangidwa kuti azindikire SARS-CAV-2 mu zitsanzo. Masakani omaliza komanso osakanikirana omaliza omwe adakonzedwa ndikuwonjezera 5 μl ya eluent mpaka 20 μl ya master kusakanikirana ndi gawo lomaliza la 25 μl. Kukwezedwa ndi kuwunika kunachitika munthawi yomweyo pa chida cha Abi 750024 cha PCR.
The Orf1a / B ndi N IRS yapezeka kuti agwiritse ntchito Sansati Biotech NCOV-2019 Nyukiliya ya Asid Dialutic Kit (Fluorescent PCR yodziwika). Konzani ma profices a Centers iliyonse posankha njira ya banja la Orf1a / B njira ya rox ya NE RE. Kupita kwa Kit, GUOME NDI MALO OGWIRITSIRA NTCHITO ZOPHUNZITSIRA: Konzani 30 μl Carsacer Mistem ndi 20 μl adayikidwa zitsanzo zodziwika / Kutsitsidwa. PCR yeniyeni PCR ABI 750025 idagwiritsidwa ntchito powonjezera / kuwonekera.
Kuyesa kwa SAV-2 BGI ndi njira yeniyeni ya Rrt-PCR pakuzindikira Covid-19. Chigawo chandamale chimapezeka mu Orf1a / B Dedera la SARS-COV-2, mtundu umodzi wowona. Kuphatikiza apo, mtundu wa dziko la munthu β-Actin ndi Gerctriner yokhazikitsidwa mkati. Kusankhidwa kwa mbuyeyo kumakonzedwa ndi kusakaniza 20 μl kusakanikirana kopambana ndi 10 μl ya RNA yotulutsidwa ndi RNA Yachitsime pachitsime. Chida cha ABI 7500 cha PCr PCR chidagwiritsidwa ntchito pakukulitsa ndi kudziwika. Masinthidwe onse a nuclectic acidic, pcr othamanga aliyense pakali aliyense, ndipo kutanthauzira kwa zotsatira zinachitika malinga ndi malangizo a wopanga (Gome 3).
Mu kuwerenga kofananira kumeneku, sitinagwiritse ntchito njira yodziwika bwino kuti titsimikizire mgwirizano wambiri (wothira, wosalimbikitsa, komanso wokwera kwambiri) ndi magawo ena oyerekeza owunika. Kuyerekeza kulikonse koyesa kunachitika ndi Cer, mu phunziroli ma crs adakhazikitsidwa ndi lamulo "labwino lililonse" ndipo zotsatira zake zidatsimikizika, osati zoyeserera zingapo. Kuphatikiza apo, pankhani ya kufala kwa Covid-19, zotsatira zabodza zabodza zimakhala zowopsa kuposa zotsatira zabodza. Chifukwa chake, kunena kuti "zabwino" molondola kuchokera ku CRS zotsatira molondola, kuyesa konse kwa lingaliro kuyenera kukhala kotsimikizika, kutanthauza kuti zotsatirapo zingapo zabwino zingakhale zochokera ku Euay. Chifukwa chake, mwazotsatira zinayi kapena zoyeserera ziwiri kapena zingapo zomwe zimapereka zotsatira zomwezo zimawonetsera zabwino kapena zoipa18,27.
Zambiri zimatengedwa pogwiritsa ntchito mitundu yopanga deta, kulowa kwa deta ndi kusanthula kwa data kumachitika pogwiritsa ntchito pulogalamu yowerengera ndi SPS SPS 23.0 pofotokozera. Pangano lolakwika, loipa, ndi mgwirizano wonse lidasanthulidwa, ndipo alama a Kappa adagwiritsidwa ntchito kudziwa kuchuluka kwa mgwirizano wa njira iliyonse yokhala ndi ma cr. Miyezo ya Kappa imatanthauziridwa motere: 0.01 mpaka 0.20 mpaka 0.41 mpaka Pangano Lalikulu, 0.61-0.80.
Chilolezo chamakhalidwe chidapezeka kuchokera ku yunivesite ya Addis Ababa ndi ma protocol onse a phunziroli adavomerezedwa ndi zipatala za Indiretute. Nambala ya Chilolezo cha EFI ya EFI ndi Ephi / IRB-279-2020. Njira zonse zidagwiritsidwa ntchito malinga ndi malangizo ndi zitsogozo za umunthu wa ku Itiyopiya za dziko lonse zochizira Covid-19. Kuphatikiza apo, kuvomereza kudziwitsidwa kwa chidziwitso kunapezeka kuchokera kwa onse omwe amaphunzira asanatenge nawo gawo phunzirolo.
Zambiri zomwe zapezedwa kapena kusanthula phunziroli zikuphatikizidwa m'nkhani yofalitsayi. Zambiri zomwe zimathandizira pa kafukufukuyu zikupezeka kuchokera ku Woyambitsa Varsive Pempho Loyenera.
Bungwe Loyanganira Thanzi Padziko Lonse la Pansi. Malangizo a njira zoyesera la labotale za Covid-19: Kuwongolera kwa Intery, March 21, 2020 Ayi. Ndani / 2019-NCAV / 2020.1 (ndani, 2020).
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