PCR Pearthhoothouts: Mafunso Omwe Amafunsidwa Kwambiri ndi Mayankho

Polymemeseset Intetion (PCR) Openda ndi zida zofunikira mu biology cabilogy carology, kulola ofufuza kuti apitilize ntchito kuyambira kafukufuku wa ma genetical diactic matenda. Komabe, monga chida chilichonse chovuta, pcr perler amatha kukumana ndi mavuto omwe amakhudza momwe amagwirira ntchito. Nkhaniyi ikufotokozera mafunso ena okhudzaPCR CareKuvutitsa komanso kumapereka njira zothandizira pamavuto wamba.

1. Kodi chitani changa changa sichikuwalitsa?

Chimodzi mwazomwe zimakumana ndi ogwiritsa ntchito ndizolephera kwa PCR yomwe ikuchitika kuti ikukweze. Izi zitha kufotokozedwa kwa zinthu zingapo:

Kupanga kolakwika kolakwika: Onetsetsani kuti primers anu ndi ena mwatsatanetsatane ndipo ali ndi kutentha koyenera (TM). Gwiritsani ntchito mapulogalamu a Promer Kormer kuti mupewe kukhazikika.

Sipira yokwanira DNA: Tsimikizirani kuti mukugwiritsa ntchito template wokwanira. Zochepa kwambiri zimapangitsa kuti zikhale zopanda mphamvu kapena zowonjezera.

Zoletsa mu sampuli: zodetsa zomwe zimayipitsa zitha kulepheretsa PCR. Ganizirani kuyeretsa DNA yanu kapena kugwiritsa ntchito njira yodulira.

CHENJEZO: Onani kapangidwe kanu kakang'ono, onjezani ma template, ndipo onetsetsani kuti zitsanzo zanu zilibe zoletsa.

2. Chifukwa chiyani pcr yanga ili yolakwika kukula kolakwika?

Ngati kukula kwanu kwa PCR sikukuyembekezeredwa, kungasonyeze vuto ndi zomwe mungagwiritse ntchito.

Kutsitsidwa kwatsatanetsatane: Izi zitha kuchitika ngati primer imayamba ku malo osakonzekera. Onani tanthauzo la olemba pogwiritsa ntchito chida monga kuphulika.

Kutentha kolakwika: Ngati kutentha kotsika kwambiri ndi kochepa kwambiri, kopanda tanthauzo kumatha. Kuthana kwa kutentha kwa kutentha ndi gradient pcr.

Njira Yothetsera: Tsimikizani Producer kuti muchepetse kutentha kwa PCR.

3.. Kodi nditani?

Mauthenga Olakwika pa PCr Persezer amatha kukhala owopsa, koma nthawi zambiri amapereka mavuto.

Zithunzi zowoneka bwino: Onetsetsani kuti PCR Perchzer imakhazikika molondola. Macheke okhazikika komanso matchulidwe osinthika ndi ovuta kupeza zotsatira zolondola.

Gulu la mapulogalamu: Nthawi zina, mapulogalamu a mapulogalamu amatha kuyambitsa mavuto. Yambitsaninso kompyuta yanu ndikuyang'ana zosintha mapulogalamu.

Yankho: Fotokozerani buku la ogwiritsa ntchito pa code yolakwika ndikutsatira njira zolimbikitsira zomwe zikulimbikitsidwa. Kukonza pafupipafupi kungalepheretse mavuto ambiri.

4. Chifukwa chiyani PCR yanga ili yosagwirizana?

Zotsatira zosagwirizana ndi PCR zitha kukhala zokhumudwitsa pazifukwa zingapo:

Khalidwe labwino: Onetsetsani kuti ma enternt onse, kuphatikiza ma enzymes, ophatikizika, ndi ma dnts, ndi atsopano komanso apamwamba kwambiri. Zowongolera kapena zowonongeka zimatha kusiyanasiyana.

Thermal cycler carcration: Makonda osagwirizana nawo angakhudze njira ya PCR. Nthawi zonse onani kambuku wa mankhwala osokoneza bongo.

Njira Yothetsera: Gwiritsani ntchito ma reagents apamwamba kwambiri ndikukulitsani kwa wozungulira wanu pafupipafupi kuti muwonetsetse zotsatira zosasinthasintha.

5. Kodi mungasinthe bwanji PCR yomwe ikugwira?

Kuwongolera luso la pcr kumatha kubweretsa zokolola zapamwamba komanso zotsatira zodalirika.

Dziwani Zinthu Zomwe Zimachitika: Kuyesa Kugwiritsa Ntchito Zosiyanasiyana za Primers, template DNA ndi MGCL2. Chiwonetsero chilichonse cha PCR chitha kuganiza kuti zinthu zapadera zizichita bwino.

Gwiritsani ntchito michere yayitali: Ngati kulondola, lingalirani pogwiritsa ntchito chodalirika kwambiri DNA polmerase kuti muchepetse zolakwika mu makutu.

Njira Yothetsera: Chitani zoyeserera kuti mupeze malo abwino kwambiri pa chikhazikitso chanu cha PCR.

Powombetsa mkota

Kuvutitsa aPCR CareItha kukhala ntchito yovuta, koma kumvetsetsa mavuto ambiri ndi mayankho awo kumatha kukulitsa luso lanu la PCR. Potha kuthetsa mavuto omwewa, ofufuza amatha kusintha zotsatira za PCR ndikuwonetsetsa zotsatira zodalirika m'mitundu yazomwe zimagwiritsidwa ntchito molecular. Kukonza pafupipafupi, kusankha mosamala kwa ma reagents, ndi kukhathamiritsa kwa mikhalidwe ndi njira zopezera kusanthula kwa PCR.