Nkhani - Tsegulani chivindikiro ndikuyang'ana - Nsomba Zazikulu 40mins matenda a nkhumba ozindikira mwachangu

Tsegulani chivindikiro ndikuyang'ana - Nsomba Zazikulu 40mins matenda a nkhumba ozindikira mwachangu

Njira yatsopano yodziwira matenda a nkhumba kuchokera ku Big Fish yakhazikitsidwa. Mosiyana ndi miyambo madzi kudziwika reagents kuti amafuna Buku kukonzekera anachita kachitidwe, izi reagent utenga mokwanira chisanadze amaundana zouma microsphere mawonekedwe, amene akhoza kusungidwa firiji popanda kukhudza ntchito ya reagent. Pozindikira, ma nucleic acid okha omwe amachotsedwa ayenera kuwonjezeredwa potsegula chivindikirocho. Pambuyo pake reagent itasungunuka kwathunthu, imatha kuyesedwa pamakina. Pophatikizidwa ndi makina opangira ma nucleic acid ndi zida za Big Fish, Big Fish yakhazikitsa njira yodziwira matenda a nkhumba kwa mphindi 40. Ndi mapulojekiti akuluakulu asanu ndi limodzi ozindikiritsa kuphatikizapo Blue Ear, Pseudorabies, Swine Fever, Circovirus, Non Circovirus, ndi Porcine Influenza, zimangotenga pafupifupi mphindi 40 kuti amalize ndondomeko yonse ya PCR fluorescence quantitative detective kuchokera ku sampuli processing mpaka zotsatira zodziwika.

Njira Yothetsera

1. Kutulutsa kothandiza - zitsanzo zingapo zitha kukonzedwa mumphindi 10
Pogwiritsa ntchito Big Fish universal nucleic acid m'zigawo ndi kuyeretsa reagent pamodzi ndi kwathunthu basi nucleic asidi m'zigawo ndi kuyeretsa chida, nucleic asidi m'zigawo akhoza kuchitidwa pa zitsanzo zosiyanasiyana (kuphatikizapo magazi athunthu, seramu, madzi a m'magazi, swabs chilengedwe, swabs m'kamwa, swabs ndowe, etc.) mkati pafupifupi mphindi 10 popanda kufunikira kwa zitsanzo zovuta pretreat. Pambuyo pakutsitsa kwachitsanzo, imatha kuchotsedwa pamakina.

2. Kukulitsa kwachangu -30 min quantification ya fluorescence mwachangu
Pogwiritsa ntchito Big Fish BFOP-1650 Fluorescence Quantitative PCR Analyzer, mawonekedwe akutchire a Big Fish amaundana zowuma zowumitsa amatha kuyatsidwa. Kuphatikiza kwa ma reagents owumitsa ndi kuwunika mwachangu kwa mphindi 30 kumatha kukwaniritsa chivundikiro chotseguka komanso kuyezetsa patsamba.

3. Kusanthula kwanzeru - ntchito yofunika, kusanthula kodziwikiratu
The Big Fish BFOP-1650 fluorescence quantitative PCR analyzer sifunikira makonda ovuta a pulogalamu. Dinani "Yambani" kuti zinthu zonse zodziwikiratu ziyambe kudziwika. Mukamaliza kukulitsa, imangopanga chiweruzo chabwino ndi cholakwika popanda kusanthula deta pamanja.

Nthawi yotumiza: Jun-21-2025