Momwe mungasankhire chozungulira chotenthetsera choyenera pazosowa zanu pa kafukufuku

Thermal cyclersndi zida zofunika kwambiri pankhani ya biology ya maselo ndi kafukufuku wa majini. Kachipangizo kameneka kamatchedwanso PCR (polymerase chain reaction), kachipangizo kameneka n’kofunika kwambiri kuti DNA ikwezeke, n’kupangitsa kuti ikhale mwala wapangodya wa zinthu zosiyanasiyana monga kupanga ma cloning, sequencing ndi gene expression analysis. Komabe, pali zosankha zambiri pamsika kotero kuti kusankha woyendetsa bwino wamafuta pazosowa zanu zofufuza kungakhale ntchito yovuta. Nazi zina zofunika kuziganizira popanga chisankho.

1. Mvetserani zomwe mukufuna kufufuza

Musanadumphire m'magulu osiyanasiyana oyendetsa matenthedwe, ndikofunikira kuti muwunikire zomwe mukufuna pakufufuza. Ganizirani za mtundu wa kuyesa komwe mudzachita. Kodi mukugwiritsa ntchito PCR yokhazikika, kuchuluka kwa PCR (qPCR), kapena pulogalamu yapamwamba kwambiri? Iliyonse mwa mapulogalamuwa angafunike mawonekedwe osiyanasiyana ndi kuthekera kwa woyendetsa matenthedwe.

2. Kutentha Kusiyanasiyana ndi Kufanana

Kutentha kwamtundu wa thermal cycler ndi chinthu chofunikira kwambiri. Mapulogalamu ambiri a PCR amafunikira sitepe ya denaturation pafupifupi 94-98 ° C, sitepe yochepetsera pa 50-65 ° C, ndi sitepe yowonjezera pa 72 ° C. Onetsetsani kuti chozungulira chotenthetsera chomwe mwasankha chimatha kuthana ndi kutenthaku komanso kuti kutentha kumagawidwa mofanana mu module yonse. Kusafanana kwa kutentha kungakhudze kafukufuku wanu poyambitsa zotsatira zosagwirizana.

3. Mtundu wa block ndi mphamvu

Ma cyclers otenthetsera amabwera m'njira zosiyanasiyana, kuphatikiza mbale 96-zitsime, mbale 384-zitsime, ngakhale mbale 1536-chitsime. Kusankhidwa kwa mtundu wa block kuyenera kufanana ndi zosowa zanu. Ngati mukuchita zoyeserera zapamwamba, mungafunike mawonekedwe okulirapo. Mosiyana ndi zimenezi, pazoyesera zazing'ono, mbale ya 96-chitsime ikhoza kukhala yokwanira. Kuphatikiza apo, ganizirani ngati mukufuna ma module osinthika amitundu yosiyanasiyana, chifukwa izi zitha kukulitsa kusinthasintha kwa kafukufuku wanu.

4. Liwiro ndi Mwachangu

M'malo ochita kafukufuku ofulumira masiku ano, nthawi ndiyofunikira. Yang'anani chozungulira chotenthetsera chokhala ndi mphamvu zotenthetsera mwachangu komanso zoziziritsa. Mitundu ina yapamwamba imatha kumaliza kuzungulira kwa PCR mkati mwa mphindi 30, ndikufulumizitsa mayendedwe anu. Kuphatikiza apo, zinthu monga kufulumira kapena kutentha kwachangu kumawonjezera magwiridwe antchito, kukulolani kuti mupange zitsanzo zambiri munthawi yochepa.

5. User Interface ndi Mapulogalamu

Mawonekedwe osavuta kugwiritsa ntchito ndi ofunikira kuti agwire bwino ntchito. Yang'anani chozungulira chotenthetsera chokhala ndi zenera logwira mwachilengedwe, zosankha zosavuta zamapulogalamu, ndi ma protocol omwe adakhazikitsidwa kale. Mitundu yapamwamba imathanso kubwera ndi mapulogalamu omwe amalola kuyang'anira nthawi yeniyeni ndi kusanthula deta, zomwe zimakhala zopindulitsa kwambiri pamapulogalamu a qPCR. Onetsetsani kuti pulogalamuyo ikugwirizana ndi machitidwe anu omwe alipo ndipo imatha kuthana ndi zomwe mukufuna.

6. Malingaliro a Bajeti

Ma cyclers otenthetsera amasiyana kwambiri pamtengo, choncho ndikofunikira kukhala ndi bajeti musanayambe kugula. Ngakhale zingakhale zokopa kuti mupite ndi njira yotsika mtengo kwambiri, ganizirani kufunika kwa nthawi yaitali kuyika ndalama pamakina apamwamba omwe amakwaniritsa zosowa zanu zofufuza. Musaganizire za mtengo wogula woyambirira, komanso mtengo wazinthu, kukonza, ndi kukweza komwe kungatheke.

7. Thandizo la Opanga ndi Chitsimikizo

Pomaliza, ganizirani kuchuluka kwa chithandizo ndi chitsimikizo choperekedwa ndi wopanga. Woyendetsa matenthedwe odalirika ayenera kupereka chitsimikizo chokwanira ndikukhala ndi chithandizo chamakasitomala pakuthana ndi mavuto ndi kukonza. Izi zimakupulumutsirani nthawi ndi chuma m'kupita kwanthawi.

Pomaliza

Kusankha choyeneramatenthedwe cyclerchifukwa chosowa kafukufuku wanu ndi chisankho chofunikira chomwe chingakhudze kupambana kwa kuyesa kwanu. Poganizira mosamala zomwe mukufuna, kuchuluka kwa kutentha, mawonekedwe a module, liwiro, mawonekedwe a ogwiritsa ntchito, bajeti, ndi thandizo la opanga, mutha kupanga chisankho chodziwitsa chomwe chingakulitse luso lanu lofufuza ndikupeza zotsatira zodalirika. Kuyika nthawi muzosankhazi kudzakuthandizani kukhala ndi luso komanso luso la ntchito yanu yasayansi.