Kuyesa kwa DNA Methylation kumaphatikizidwa ndi mafoni a mafoni owunikira zotupa ndi leukemia ndikulondola kwa 90.0%!

Kuzindikira khansa yoyambirira ya khansa yamadzimadzi ndi njira yatsopano yopezera khansa ndi US National khansa itakhazikitsidwa m'zaka zaposachedwa, ndikuwona kukhala ndi khansa yoyambirira kapena yotupa. Zakhala zikugwiritsidwa ntchito kwambiri ngati buku la Biogarker pozindikira matenda osiyanasiyana a zilonda zambiri, kuphatikiza khansa yam'mapapo, zotupa zam'mimba, gliomas ndi zotupa zamatsenga.

Kutuluka kwa nsanja kuti muzindikire mawonekedwe a metyction

Posachedwa, ofufuza apanga nsanja yosavuta komanso yachindunji ya Tystelape kukongoletsa golide nanoparticles (cyst / aunps) ophatikizidwa ndi kuwunikira koyambirira kwa zotupa zingapo. Kuwonetsera koyambirira kwa leukemia kumatha kuchitidwa mkati mwa mphindi 15 pambuyo pa DNA kumachotsa mtundu wa magazi, molondola 90.0%. Mutu wankhani ukudziwika bwino khansa DNA m'magazi amunthu pogwiritsa ntchito anyusiki ogwiritsa ntchito a Cysteimine ndi makina ophunzirira maphunziro.

Chithunzi 1. Phukusi losavuta komanso lachangu la kuwunika kwa khansa kudzera pa cyst / aunips itha kuchitika m'njira ziwiri zosavuta.

Izi zikuwonetsedwa pa Chithunzi 1. Choyamba, njira yothetsera matendawa idagwiritsidwa ntchito kusungunula zidutswa za DNA. Cyst / Aunps adawonjezeredwa ku yankho losakanizika. Zabwinobwino komanso zoyipa DNA zimakhala ndi zinthu zosiyanasiyana methyty, zomwe zimapangitsa DNA ndi misonkhano yosiyanasiyana. DNA yachilendo kwabwino kwambiri ndipo pamapeto pake aggrectites cyst / Aunps, omwe amachititsa kuti chizolowezi chofiyira, kotero kuti kusintha kwa utoto kufinya kumatha kuwonedwa ndi maso. Mosiyana ndi izi, mawonekedwe a khansa yapadera ya khansa DNA imatsogolera pakupanga magulu akulu akulu a DNA.

Zithunzi za 96-Welly zidatengedwa pogwiritsa ntchito kamera ya smartphone. Cancer DNA idayesedwa ndi smartphone yokhala ndi maphunziro amakina poyerekeza ndi njira zowoneka bwino.

Kuwunika khansa mu zitsanzo zenizeni za magazi

Kuwonjezera zofunikira papulatifomu yomvekera, ofufuzawo adagwira ntchito yomwe imadziwika bwino pakati pa nthawi zonse pakati pa anthu wamba komanso khansa. Mitu ya methylation pa CPG imayang'anira mitundu ya jen. Pafupifupi mitundu yonse ya khansa, kusintha mu DNA methylation ndipo motero posonyeza majini omwe amalimbikitsa phula ku Tamourigeniis adawonedwa.

Monga chitsanzo kwa khansa zina zokhudzana ndi DNAMESTTER, ofufuzawo adagwiritsa ntchito zitsanzo zamagazi ndi odwala athanzi komanso zowongolera zotheka kufufuza mphamvu ya majeremusi a meukaec. Ndondomeko ya methypec discarker osati yongosintha njira zowunikira za leukemia zomwe zidalipo, komanso zimawonetsa kuthekera kofikira mobwerezabwereza makeke ogwiritsa ntchito mawu osavuta komanso owongoka.

DNA yochokera ku zitsanzo za magazi ndi 31 leukemia odwala ndi anthu 12 athanzi adasanthula. Monga taonera m'bokosi lomwe ili pa Chithunzi 2A, omvera a khansa ya khansa (ΔA650 / 525) anali otsika kuposa a DNA kuchokera pa zitsanzo zabwinobwino. Izi zinali makamaka chifukwa cha Hydrobicicicity yolimbikitsidwa yomwe imatsogolera kuphatikizika kwa khansa DNA, yomwe idaletsa kuphatikizika kwa cyst / anyunps. Zotsatira zake, nanoparticles awa adabalalika kwathunthu m'chigawo chakunja cha khansa, yomwe idayambitsa kubalaku kwa cyst / aunps adsorbed pazabwinobwino komanso khansa DNARGORE. Ma curves a Roc adapangidwa ndikusintha panjira yotsika mtengo yaying'ono ya ΔA650 / 525 mpaka mtengo wokwanira.

Chithunzi 2. (a) Malingaliro Ochepa a CYST / Aunsps Oonetsa Kukhalapo kwabwinobwino (buluu) ndi khansa (yofiyira) DNA pansi pa Mikhalidwe Yokwezeka

(Da650 / 525) ya bokosi; (b) Kusanthula kwa Roc ndi kuwunika mayeso a mayeso ozindikira. (c) Kusokonezeka Matrix pakuzindikira kwa odwala wamba komanso a khansa. .

Monga taonera Chithunzi 2B, malo omwe ali pansi pa roc curve (auc = 0.9274) omwe adapezeka kuti amveketse sewero la seweroli lamphamvu komanso mwachindunji. Monga tikuwonera kuchokera m'bokosi lomwe, mfundo yotsika kwambiri yomwe ikuimira gulu la DNA silikhala olekanitsidwa ndi mfundo yapamwamba kwambiri yomwe ikuimira gulu la DNA DNA Gulu la DNA DNAS; Chifukwa chake, kulembetsa kwamilandu kunagwiritsidwa ntchito kusiyanitsa magulu a khansa komanso khansa. Popeza kukhazikitsidwa kwa zosintha zodziyimira pawokha, kumatinso kuthekera kwa chochitika, monga khansa kapena gulu wamba. Zosiyanasiyana zosintha pakati pa 0 ndi 1. Zotsatira zake ndiye kuthekera. Tidatsimikiza kuti kuthekera kwa khansa (p) Kutengera ΔA650 / 525 motere.

komwe B = 5.3533, w1 = -6.965. Kwa gulu la zitsanzo, kuthekera kwa 0,5 kuwonetsa zitsanzo zabwinobwino, pomwe mwayi wa 0,5 kapena kupitilira ukuwonetsa chitsanzo cha khansa. Chithunzi 2c chikuwonetsa chisokonezo cha matrix omwe adapangidwa kuchokera ku tchuthi-chokhacho chomwe chidagwiritsidwa ntchito kutsimikizika, chomwe chidagwiritsidwa ntchito kutsimikizira kukhazikika kwa njira yogawika. Chithunzi 2D chimafotokoza mwachidule kuzindikira kuwunikira njira, kuphatikiza, kuphatikiza, kusanja kwamtengo wapatali (PPV) ndi mtengo wolosera (NPV).

Smartphone-radiosers

Kuti athe kuyezetsa zitsanzo mosavuta popanda kugwiritsa ntchito zowoneka bwino, ofufuzawo adagwiritsa ntchito luntha la zojambula (AI) kutanthauzira mtundu wa yankho ndikusiyanitsa anthu wamba komanso khansa. Popeza uwu, masomphenya a makompyuta anali kugwiritsidwa ntchito kutanthauzira mtundu wa cyst / aunps yankho labwinobwinobwino. Luntha lanzeru limatha kuchepetsa mtengo ndikuwongolera mwayi wotanthauzira mtundu wa nanoparlecle mayankho, ndipo popanda kugwiritsa ntchito njira iliyonse yama smartphone. Pomaliza, mitundu iwiri yophunzirira, kuphatikizapo nkhalango yachilendo (RF) ndi makina othandizira vector (SVM) adaphunzitsidwa kupanga mitunduyo. Onse awiri a RF ndi SVM ndi SVM moyenera adatsimikiza zitsanzo zabwino komanso zoyipa ndi kulondola kwa 90.0%. Izi zikusonyeza kuti kugwiritsa ntchito luntha laukadaulo mu mabizinesi okhudzana ndi mafoni ndikotheka.

Chithunzi 3. (a) Gulu landamale la yankho lomwe lalembedwa pokonzekera zitsanzo za fano loti likwaniritsidwe. (b) Chithunzi cha Chitsanzo chojambulidwa panjira yokwanira. .

Kugwiritsa ntchito cyst / aunps, ofufuza apanga bwino papulogalamu yosavuta ya mitu ya methylate ndi sensor yomwe imatha kusiyanitsa DNA kuchokera ku khansa DNA yomwe imagwiritsa ntchito magazi a Leukemia. Sensor yotukuka yomwe DNA idachokera ku zitsanzo zenizeni za magazi zidatha mwachangu komanso zimangodziwa bwino khansa yochepa kwambiri. Kuti athe kuyererera kwachitsanzo kosavuta kwambiri pochotsa kufunika kwa mawonekedwe a spectophotometer, maphunziro amakina amagwiritsidwa ntchito kutanthauzira mtundu wa yankho komanso kudzipatula pa 90.0%.

Referew: Doi :.1039 / D2RA05725E