Phunziro Lodzipereka: Tekisi ya PCR ya PCDNA Methylation Technology imatsegulira nthawi yatsopano ya MRD

Posachedwa, Jama Omeclogy (ngati 33.012) yofalitsa maphunziro ofunikira [1] Chotupa DNA CHATEYPATER ndi Stratition Stratation) ". Phunziroli ndi kafukufuku woyamba wa anthu padziko lonse lapansi kugwiritsa ntchito ukadaulo wa PCRNARent Collenology yolosera za MRDERE NDI CHITSANZO CHOSAVUTA KUSINTHA KWA ADZAKHALA NDI MALO OGWIRITSIRA NTCHITO NDIPONSO KUSINTHA KWAULERE NDIPO KUSINTHA KWAULERE NDI MOYO WABWINO. Kafukufukuyu adawunikiranso kwambiri polemba magaziniyi komanso akonzi ake, ndipo atchulidwa kuti pepala lalikulu m'magazini ino, ndipo Pulofesa Raiz Apriz-Bafestor A Ruiz And Ajay Ankay Goel Goise adapemphedwa kuti awunikenso. Kafukufukuyu adanenedwanso ndi Genomeweb, media yotsogola ku United States.

Khansa ya colorectial (CRC) ndi chotupa chododometsa cha m'mimba ku China. 2020 International International Kafukufuku Wokhudza khansa (IARC) kuwonetsa kuti milandu ikuluikulu ya anthu pafupifupi 1/3 ya anthu pafupifupi 1/3 ya dziko lapansi, ndikudumphira kachiwiri ku China; 2865 Imfa ya Imfa ya 1/3 ya dziko lapansi, ikugwira ntchito yachisanu yomwe imayambitsa khansa ku China. Chachisanu chomwe chimayambitsa kufa ku China. Ndizosangalatsa kuti pakati pa odwala omwe adapezeka, TNM Stations I, II, III ndi IV pali 18.6%, 42.5%, 30.7% motsatana. Opitilira 80% ya odwala ali mtunda wapakati komanso mochedwa, ndipo 44% a iwo ali ndi metastasent metastases kupita ku chiwindi ndi m'mapapo, kuwononga thanzi la okhalamo ndikuyambitsa chuma chambiri. Malinga ndi ziwerengero za National Cancer Center, kuwonjezeka kwachuma pachaka ku China ku China ndi pafupifupi 6.9% mpaka 9.2% Odwala khansa akuvutika ndi matendawa komanso mogwirizana ndi mavuto azachuma [2].
Ninsta makumi asanu ndi anayi pa zotupa khansa ya coloretal itha kuchotsedwa mwadzidzidzi, ndipo chotupa choyambirira chikapezeka, chokweza chazaka zisanu pambuyo pa opareshoni zambiri pambuyo pa 30%. Kupulumuka kwa zaka zisanu za khansa yadzikoli kwa anthu aku China ndi 90.1%, 72.6%, 53.8%, II, III ndi IV, motsatana.
Matenda otsalira ochepa (MRD) ndi chifukwa chachikulu chomwe chimachotsa chotupa chitatha chithandizo chamankhwala. M'zaka zaposachedwa, ukadaulo wa Mrd wa zotupa zolimba zapita patsogolo, ndipo maphunziro angapo owoneka bwino atsimikizira kuti postoperative abweretse chiopsezo cha khansa ya postoreal Kuyesa kwa CTDNA kuli ndi zabwino zokhala zopanda pake, zosavuta, mwachangu, ndi zitsanzo zambiri komanso kuthana ndi chotupa chizikhala chotupa.
Malangizo a US Nccn a khansa ya Coloni ndi malangizo a khansa ya ku China kuti akwaniritse zomwe zimachitika m'zovuta za Conlotherast Komabe, mafilimu ambiri omwe alipo amayang'ana paukadaulo wa CTDNA potengera ukadaulo wapamwamba (NGs), zomwe zili ndi zovuta, nthawi yayitali yotsogolera, komanso kuchepa kwambiri pakati pa odwala khansa.
Pankhani ya steji ya III Colorectal khansa, CGS-CRDNA yowunikira mwamphamvu imawononga $ 10,000 kwa maulendo amodzi ndipo pamafunika nthawi yodikirira mpaka milungu iwiri. Ndi chiyeso cha methylation methylation mu kafukufukuyu, a Coloboiq ®, odwala amatha kukhala ndi ma ctdna kuwunikira khumi mwa khumi mwa mtengo wake ndikupeza lipoti mochepa ngati masiku awiri.
Malinga ndi milandu yakale ya 560,000 ya khansa ya coloelel ya ku China chaka chilichonse, odwala matenda ali ndi khansa ya II-III.
Zitha kuwona kuti zotsatira zofufuzira zili ndi tanthauzo lofunikira lasayansi komanso lothandiza. Kudzera mu maphunziro apamwamba kwambiri azachipatala, zatsimikizira kuti ukadaulo wa PCR unagwiritsidwanso ntchito kulosera kwa khansa yobwereza ndi kuwunikiranso, kugwira bwino ntchito, kumathandizira bwino odwala khansa. Phunziroli limachokera ku Colounaq ®, mayeso a metylation metylation khansa yopangidwa ndi Kuny, yemwe maphunziro ake azachipatala omwe amapezeka moyambirira komanso matenda atsimikiziridwa ndi kafukufuku wapakati.
Gastroennology (LE33.88), mtolankhani wapadziko lonse lapansi mu 2021, chipatala chambiri cha a Coloaiqan molumikizana Khansa yapadera, ndipo poyamba idafufuzanso zomwe zimawunikiranso zomwe zingagwiritsidwe ntchito kuwunika kwa proplosis.

Kuti mutsimikizire kugwiritsa ntchito mankhwala a CTDNA methylation stratation, kuwongolera chithandizo chobwereza, ndipo patatha miyezi itatu yochita opaleshoni, ndipo postoperative actruction kuyezetsa magazi mwamphamvu.
Choyamba, kunapezeka kuti kuyesa kwa CTDNA kumatha kuneneratu chiopsezo choyambiranso khansa ya khansa kwa odwala, onse opita nalo. Odwala a CTDNA-abwino anali ndi kuthekera kwakukulu kwa zinthu zakale zoyambira kuposa Ctdna - odwala olakwika 22.0%> 4.7%). Kuyeserera koyambirira kwa CTDna kunanenedweratu zoopsa: Mwezi umodzi pambuyo potsata odwala, odwala abwino a CTDNA anali ndi zaka 17.5 nthawi zambiri mpaka kalekale kuposa odwala olakwika; Gululi linapezanso kuti kuphatikiza Ctdna ndi Cea kuyesera kosintha pang'ono pofufuza (auc = 0.849) Kuyesa kokha poyerekeza ndi Ctdna yekha (AUC = 0.839).
Matenda achipatala ophatikizidwa ndi ziwopsezo zomwe zili pachiwopsezo chachikulu cha odwala khansa, ndipo paradigm yapano, ndipo pakufunikanso zida zothandizirana ndi chithandizo. Kutengera izi, gulu lomwe lili ndi odwala a III Colorectol khansa yosiyanasiyana kutengera zoopsa (T4 / N2) ndi nthawi yochepa (3/6). Kusanthula kumeneku kunapezeka kuti odwala omwe ali ndi anzawo oopsa a Ctdna-chabwino anali ndi kutsika kochepa kwambiri ngati alandila miyezi isanu ndi umodzi; Munthawi yochepa kwambiri ya odwala a Ctdna-abwino, kunalibe kusiyana kwakukulu pakati pa njira zolumikizirana ndi zomwe wodwala amachita; pomwe odwala a CTDNA-oyipa anali ndi vuto labwino kwambiri kuposa odwala a CTDNA - nthawi yopanda ntchito (RFs); Gawo I ndi Lotsika Lotsika khansa ya II Colorecal All CTDNA - odwala oyipa sanathenso kutsata zaka ziwiri; Chifukwa chake, kuphatikiza kwa Ctdna ndi mawonekedwe azachipatala akuyembekezeka kukwaniritsa gawo la chiopsezo ndikulosera bwino.

Chithunzi 1. Plasma CTDNA Kusanthula ku Pom1 chifukwa chodziwitsa kwambiri khansa
Zotsatiranso za kuyesa kwa CTDNA kuwonetsa kuti chiopsezo choyambira kusinthidwanso mwamphamvu kwa miyezi 20 (Chithunzi 3b), kupereka mwayi wopezeka woyamba wa matenda. Kubwezeretsa matenda komanso kulowererapo kwa nthawi.

Chithunzi 2.

"Kuyesa kwamankhwala ambiri omasulira a khansa ya colorenal kuwonetsa kuthekera kwakukulu kopititsa patsogolo ntchito za khansa ya colorectiated khama, kuwongolera chithandizo komanso kuwunikira koyambirira.

Ubwino wakusankha DNAMESTTUTTER monga cholembera cha nerd pabwino pa mabiri ndikuti sizimagwiritsidwa ntchito mwachindunji mankhwala okwerera magazi, ndipo amagwiritsa ntchito zotsatira zabwino kwa magazi, ndi matenda a hematopoies.
Kafukufukuyu ndi maphunziro ena okhudzana ndi ma CTDna-rocturence omwe ali ndi chiopsezo choyambirira chobwezeretsanso khansa ya i-III
Gawo la MRD limatulutsa mofulumira ndi zinthu zingapo zokhala ndi zolimbitsa thupi komanso zingapo zochokera ku Epigenics (DNA Methylation ndi fraggnomics ndi fraggnomics ndi fraggnomics ndi fragnecics ndi fraggnomics ndi fragylaty ndi fragylaty ndi fragylat ndi scaments) ndi zingwe zowoneka bwino kapena zingwe zonse. Tikuyembekeza kuti Coloaiaiq ® ikupitilira maphunziro apamwamba kwambiri ndipo imatha kukhala chizindikiro chatsopano cha kuyezetsa kwa MRD yomwe imaphatikiza kupezeka, magwiridwe antchito komanso osowa kwambiri ndipo amatha kugwiritsidwa ntchito kwambiri pamachitidwe azachipatala. "
Maumboni
[1] Jama oncol. 2023 Apr 20.
[Chithunzi patsamba 2] "Chipsinjo cha matenda a khansa ku China cha anthu aku China: chasintha m'zaka zaposachedwa? , News Insuation ya Epidemiology, Vol. 41, No. 10, Okutobala 2020.
[3] Tarazona N, Gimeno-Vagardla F, Gamaterla v, et al. Kukhazikika kwamphamvu yotsatira ozungulira ozungulira-chotupa DNA pofuna kutsatira matenda otsalira mu khansa yapadera mu khansa yapamwamba. Ann Oncol. Nov 1, 2019; 30): 1804-1812.
. Khansa imathandizira rev. 2019; 75: 1-11.