Nkhani - Bigfish FeiXu Oral Swab DNA Cleaning Kit: Yankho Lothandiza Kwambiri Pochotsa DNA ya Zitsanzo za Mkamwa

M'minda yaKuzindikira matenda m'thupi (IVD), kusanthula majini, ndi kafukufuku wa mamolekyuluzitsanzo za pakamwa—mongamankhwala ophera pakamwa, mankhwala ophera pakhosi, ndi malovu— amagwiritsidwa ntchito kwambiri poyesa nucleic acid chifukwa chakusonkhanitsa kosavuta, kosavulaza, komanso njira yosapweteka yopezera zitsanzoKomabe, zitsanzo za pakamwa nthawi zambiri zimakhala ndikuchuluka kochepa kwa ma nucleic acidndipo nthawi zambiri amaipitsidwa ndimapuloteni ndi zinthu zina zodetsaNjira zachikhalidwe zochotsera zinthu nthawi zambiri zimavutika ndinjira zovuta zogwirira ntchito, kusagwira ntchito bwino, komanso kugwiritsa ntchito zinthu zoopsa, zomwe zingasokoneze kwambiri kulondola ndi kukhazikika kwa mapulogalamu otsatira mongaPCR/qPCR ndi kutsata kwa m'badwo wotsatira (NGS).

TheBFMP06 Magnetic Bead-Based Oral Swab Genomic DNA Extraction Kit, yopangidwa ndiHangzhou Bigfish FeiXu Biotechnology, amaperekayankho lotetezeka, lothandiza, komanso lodalirikakuti mutenge DNA ya chitsanzo cha pakamwa. Ndi kapangidwe kake katsopano kaukadaulo komanso miyezo yokhwima yogwirira ntchito, zida izi zakhala chida chodalirika cha ma laboratories azachipatala komanso ofufuza.

Chida cha BFMP06 chimapangidwa mozunguliradongosolo losungiramo zinthu mwapaderapamodzi ndiMikanda ya magnetic ya hydroxyl yeniyeni ya DNA, kupanga njira yoyeretsera nucleic acid yogwira ntchito bwino kwambiri. Pambuyo poti chitsanzocho chayikidwa mu lysis buffer, zigawo za maselo zimasokonekera ndipo nucleic acids zimatulutsidwa. Magulu ogwira ntchito pamwamba pa mikanda yamaginito amasankha DNA yaulere, ndikupanga yokhazikika.maginito bead–DNA complexes.

Pansi pa mphamvu ya maginito yakunja, ma complexes amadutsamasitepe awiri olondola otsukirakuchotsa mapuloteni, mchere, ndi zinthu zina zodetsa. Pomaliza,DNA ya majini yoyera kwambiriimachotsedwa bwino pogwiritsa ntchito lution buffer.

Mafotokozedwe Akatundu

Chogulitsachi chimagwiritsa ntchito njira yapadera yosungiramo zinthu pamodzi ndimikanda ya maginito yomwe imagwirizanitsa DNA mwachindunji, zomwe zimathandiza kuti ma nucleic acid azitha kuyamwa mwachangu, kulekanitsidwa, komanso kutsukidwa. Ndi yoyenera kwambiri paKupatula DNA ya majini mwachangu komanso moyenera kuchokera ku swabs za pakamwa, swabs za pakhosi, ndi zitsanzo za malovu, pamene akuchotsa bwino mapuloteni otsala ndi mchere.

Mukagwiritsidwa ntchito limodzi ndiZida zochotsera nucleic acid zochokera ku Bigfish FeiXu, zidazi ndi zabwino kwambirikutulutsa kodzipangira okha kopangidwa ndi mphamvu zambiriDNA yoyeretsedwa ya majini ndi yakuyera kwambiri komanso khalidwe labwino kwambiri, zomwe zimapangitsa kuti ikhale yoyenera kugwiritsa ntchito mitundu yosiyanasiyana ya mapulogalamu, kuphatikizapoPCR/qPCR ndi NGS.

Zinthu Zamalonda

Mapangidwe apamwamba

Amachotsa bwino ndikuyeretsa DNA ya majini kuchokera kumankhwala ophera pakamwa, mankhwala ophera pakhosi, ndi malovukuperekazokolola zambiri komanso kuyera kwambiri.

Mwachangu komanso Mosavuta

Palibe njira zobwerezabwereza zoyezera mpweya kapena kusefa vacuum. Zimagwirizana ndi zida zotulutsira zokha, zomwe zimapangitsa kuti zikhale zabwino kwambirikukonza zitsanzo zazikulu.

Otetezeka komanso Opanda Poizoni

Palibe chifukwa chogwiritsa ntchito zinthu zoopsa zachilengedwe mongaphenol kapena chloroform.

Zida Zogwirizana

Bigfish FeiXu BFEX-16E

BFEX-32

BFEX-32E

BFEX-96

Zotsatira Zoyesera

Zitsanzo za swab ya pakamwa (yoviikidwa mu400 μL njira yosungira) ndi zitsanzo za malovu (Malovu a 200 μL + yankho losungira la 200 μL) zinakonzedwa pogwiritsa ntchitoBigfish FeiXu Oral Swab Genomic DNA Cleaning KitDNA inasowa mu70 μL chosungiramo mankhwalandipo anafufuzidwa ndielectrophoresis ya agarose gel, monga momwe chithunzi chili pansipa chikusonyezera.