Nkhani - Kutulutsa DNA Yokha Kuchokera ku Masamba a Mpunga

Kutulutsa DNA Yokha Kuchokera ku Masamba a Mpunga

Mpunga ndi umodzi mwa mbewu zofunika kwambiri, zomwe zimapezeka m'minda ya zomera za m'madzi za banja la Poaceae. China ndi imodzi mwa malo oyamba okhala mpunga, omwe amalimidwa kwambiri kum'mwera kwa China ndi chigawo cha kumpoto chakum'mawa. Ndi kupita patsogolo kwa sayansi ndi ukadaulo, njira zamakono za biology zimagwiritsidwa ntchito kwambiri pakufufuza mpunga. Kupeza DNA ya genomic ya mpunga yapamwamba komanso yoyera kwambiri kumayala maziko olimba a maphunziro a majini otsatira. BigFish Sequence Magnetic Bead-Based Rice Genomic DNA Purification Kit imathandiza ofufuza mpunga kutulutsa DNA ya mpunga mosavuta, mwachangu, komanso moyenera.

Kiti Yoyeretsera DNA ya Mpunga

Chidule cha Zamalonda:

Chogulitsachi chimagwiritsa ntchito njira yapadera yopangira zinthu zapadera komanso mikanda yamaginito yokhala ndi zinthu zinazake zomangira DNA. Chimamanga, kunyamula, ndikulekanitsa ma nucleic acid mwachangu ndikuchotsa bwino zinthu zodetsa monga ma polysaccharides ndi ma polyphenolic compounds kuchokera ku zomera. Ndi choyenera kwambiri kutulutsa DNA ya genomic kuchokera ku minofu ya masamba a zomera. Chophatikizidwa ndi BigFish Magnetic Bead Nucleic Acid Extraction Instrument, ndi chabwino kwambiri chotulutsira zitsanzo zambiri zokha. Zinthu za nucleic acid zomwe zatulutsidwa zimakhala ndi chiyero chapamwamba komanso khalidwe labwino kwambiri, zomwe zimapangitsa kuti zigwiritsidwe ntchito kwambiri pa kafukufuku wotsatira monga PCR/qPCR ndi NGS.

Zinthu Zogulitsa:
Otetezeka komanso osakhala ndi poizoni: Palibe chifukwa chogwiritsa ntchito zinthu zoopsa zachilengedwe monga phenol/chloroform

Yopangidwa ndi makina amphamvu kwambiri: Yophatikizidwa ndi chotulutsira cha Beagle sequencing nucleic acid, imatha kutulutsa zinthu zambiri komanso yoyenera kutulutsa zitsanzo zazikulu.

Kuyera kwambiri komanso khalidwe labwino: Chogulitsa chomwe chachotsedwacho chili ndi kuyera kwambiri ndipo chingagwiritsidwe ntchito pa NGS, kusakaniza ma chip ndi kuyesa kwina.

Zida zogwirizana: BigFish BFEX-32/BFEX-32E/BFEX-96E

Nthawi yotumizira: Sep-11-2025